HBV serological characteristics between NRR samples and OBI samples of from blood donors in Hefei
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 4
Abstract
Objective To analyze the correlation of HBV serological characteristics between non-reproducible reactivity (NRR) samples and occult hepatitis B virus infection (OBI) samples for blood screening. Methods A total of 144 samples with negative ELISA (HBV, HCV and HIV test) results and reactive nucleic acid tests(NAT) were collected from January 2021 to January 2023 in Anhui Blood Center, including 92 reactive samples by TMA method (combined ID-NAT) and 52 HBV DNA reactive samples by PCR method (ID-NAT). Supplementary differential testing and ID-NAT by PCR were performed on the reactive samples of the combined ID-NAT, samples that were non-reactive by both differential testing and ID-NAT by PCR were included in the NRR group, and samples that were reactive for HBV DNA detected by either method were included in the OBI group. Supplemented with HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc tests, the differences in serological pattern and positive rate between NRR samples and OBI samples were analyzed. Results A total of 53 samples were negative for differential testing and ID-NAT and were included in the NRR group, 91 samples were detected as HBV DNA reactive in either method and were included in the OBI group. HBsAg and HBeAg were not detected by serological testing in either group. The detection rates of anti-HBs, anti-HBc and anti-HBe in the NRR group and the OBI group were 64.15% vs 47.25%, 86.79% vs 94.51%, 35.85% vs 52.75%, respectively. Comparison of serological patterns between the two groups: the most frequent pattern in the NRR group was anti-HBs (+ ) and anti-HBc (+ ) (32.08%), and the most frequent pattern in the OBI group was anti-HBe (+ ) and anti-HBc (+ ) (37.36%). Conclusion There were differences in some of the test results between the NRR samples and the OBI samples in HBV serological testing, and higher anti-HBc positive rate in the NRR samples suggests a higher possibility of HBV infection.
Authors and Affiliations
Mingrui LI, Ting WANG, Zhichao CHEN, Tingting WANG, Qing HE
Keywords
Related Articles
- EP ID EP738796
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.04.006
- Views 51
- Downloads 0
HBV serologic and virus characteristics of HBsAg negative and NAT non-discriminated samples from blood donors
Objective To investigate the HBV infection of TMA initially reactive but discriminatory test non-reactive samples(NDR) after the individual donation nucleic acid detection(ID-NAT)of TMA, and analyze its serological and m...
Investigation of rare hemoglobin-coding gene defects among Chinese population
Objective To summarize the types of rare hemoglobin-coding gene defects and their phenotypes in Chinese population, aimed at providing reference for genetic counseling of couples in childbearing age from areas with high...
Application of Z-score in retrospective analysis of external quality assessment for nucleic acid testing
[Objective] To use the Z-scores as a means of evaluating the results of external quality assessment (EQA), so as to improve the quality control management in laboratories. [Methods] Z-scores control charts were plotted b...
Hemophilia A with reduced coagulation factor Ⅺ: a case report and literature review
Objective To investigate the possible molecular pathogenesis of a child with hemophilia A accompanied by coagulation factor Ⅺ reduction by testing coagulation-related indicators and genotyping in the child and his family...
Identification of B(A) subtype and analysis of bood transfusion strategiaes
Objective To investigate the serology and genotype identification method of B (A) subtype patients. Methods Test tube method (serology) was used to confirm the clinically difficult ABO blood group samples of 3 patients w...