Healing of Guinea Pig Injures Contaminated with Pseudomonas aeruginosa by using 0.7Hz Square Pulsed Magnetic Field (New Method)
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2016, Vol 2, Issue 5
Abstract
Pseudomonas aeruginosa is considered one of the main dangerous causes of burn wound infection and known as highly resistant to antibiotics. In this work the effect of Pulsed Magnetic Field (PMF) on the growth activity of P. aeruginosa is studied. Recently on our lab it is reported that exposure of P. aeruginosa to 0.7 Hz (at resonance frequency) magnetic impulses for 1h caused 24% inhibition of bacterial cellular growth relative to the unexposed one.Dermal burns in the thigh of 25 guinea pigs were done by thermal conduction through the transfer of 1.080 kJ to circular cross sectional end of (0.760+0.0002) cm in diameter. The animals were divided into 5 equal groups (5 guinea pigs per each) namely A, B, C, D and E. Animals of group A were injured and contaminated with a swap of 1x108 CFU/ml of P. aeruginosa. Animals of group B were injured and contaminated with a swap of 1x108 CFU/ml of P. aeruginosa and treated by exposure to 0.7 Hz PMF for 3 hours at a rate of 1 h/day. Animals of group C were injured and contaminated with a swap of 1x108 CFU/ml of P. aeruginosa previously exposed to 0.7 Hz PMF for 1 hour. Animals of group D were injured without contamination and treated by exposure to 0.7 Hz PMF for 3 hours at a rate of 1 h/day. Animals of group E was left as control (injured without contamination or exposure to PMF). The results indicated significant progress in wound healing for group B and almost same healing progress for groups C, D. It was concluded that the use of 0.7 Hz PMF significantly accelerates healing of wounds contaminated with P. aeruginosa. The new method is promising and need of further work to use such fields for sterilization and/or treating contaminated wounds is a must.
Authors and Affiliations
Fadel M. Ali1 , Osoris W. G1 , N. Serag2 , A. M. Khalil3
Keywords
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