Isolation and Identification of ESBL producing Escherichia coli from diabetic patients
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2015, Vol 1, Issue 1
Abstract
A total of about 25 samples of diabetic foot infection samples were collected from the diabetic patients by using appropriate sterile cotton swabs from Government Hospital, Kanchipuram. Samples were inoculated in sterile peptone water in screw – capped tubes and transported to the laboratory within an hour. The culture was inoculated in the Nutrient agar plates and the culture obtained was further identified by classical cultural methods. The identified isolate was subjected to the antimicrobial suspectibility using standard antibiotic discs. The resistant strains were subjected to screening for Extended Spectrum Beta Lactamase production by Double disk synergy test, Disk replacement test, E-Strip test and Three dimensional test. The ethanolic seed extracts of four condiments Coriander, Cumin, Fenugreek and Pepper was prepared. The filter paper disc impregnated with each ethanolic seed extract was tested against ESBL producing Escherichia coli. Zone of inhibition of each extract were recorded. All the seed extracts were found to be effective. Among the four seed extracts, fenugreek was found to be the most effective. As a global scenario is now changing towards the use of nontoxic plant products having traditional medicinal use, development of modern drugs from these seeds should be emphasized for the control of ESBL producing Escherichia coli.
Authors and Affiliations
R. Sumathi
Keywords
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