Heamolytic activities, deoxyribonuclease production and in-vitro flouroquinolones susceptibility profile of aerobic gram positive cocci associated with acne vulgaris

Journal Title: Scientific Journal of Biological Sciences - Year 2012, Vol 1, Issue 2

Abstract

Bacteriological investigations were carried out on forty–six (46) swabbed samples from the pustular and nodulocystic skin lesions of undergraduate students with acne vulgaris using standard bacteriological methods. The fluoroquinolones susceptibility profiles, heamolytic activities, deoxyribonuclease production were determined using Kirby –Bauer disc diffusion methods, Columbia Blood Agar (CBA) and Deoxyribonuclease (DNase) agar, respectively. The results showed that all the 46 clinical samples showed positive growth, with 24 (52.2%) having single bacterium isolated. Co-infection with two bacterial species was seen in 24 (28.3%), while polybacterial growth was present in 9 (19.6%) of the samples. Staphylococcus aureus was the most prevalent aerobic Gram positive cocci associated with acne vulgaris with 32 (42.1%) occurrences, followed by Staphylococcus epidermidis 26 (34.2%) and Micrococcus spp 18 (23.9%). Sixteen of the isolates produced α – haemolysin, 25 (32.9%) produced β – haemolysin and 35 (46.1%) produced no haemolysin (γ-haemolysis). Of the 16 α – haemolysin producers, Staphylococcus aureus produced the highest 9 (56.3%), followed by Staphylococcus epidermidis 5 (31.3%) and Micrococcus spp 2(12.5%). Of the seventy – six aerobic Gram positive cocci isolated only 33 (43.4) were DNase producers, while 43 (56.6) were non-DNase producers. Staphylococcus aureus were highly sensitive to Moxifloxacin, while 26 (81.3%) were sensitive to Lomefloxacin and Gatifloxacin. Staphylococcus epidermidis and Micrococcus spp were highly sensitive to Gatifloxacin, Moxifloxacin, Lomefloxacin and Levofloxacin. The results also showed that 17 (65.4%) and 13 (50.0%) of Staphylococcus epidermidis were sensitive to Pefloxacin and Nalidixic, respectively. Consequently, this study has shown that fluoroquinolones could be drugs of choice to administer to patients with acne vulgaris.

Authors and Affiliations

O. J. Akinjogunla*| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria, O. I. Medo| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria, E. P. Philip| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria

Keywords

Related Articles

Some biological aspect of carasobarbus luteus (Heckel, 1843) in hoor al-azim wetland

The Population Dynamics and status of Hemeri (Carasobarbus luteus) in Hoor Al-azim wetland (Khuzestan provinces, Iran) were investigated to derive information required for their management. During this study from 2011 to...

Hypolipidemic properties of bauhinia rufescens in alloxan-induced diabetic rats

The present investigation was aimed at evaluating the hypolipidemic properties of methanolic leaf extracts of Bauhinia rufescens (MLEBR) on alloxan-induced diabetic rats. Alloxan was injected intraperitoneally as a singl...

Floristics and structure of fallow vegetation

Vegetation-environment (soil) was studied in fallow plots in Uyo, ranging from 0-year-old to 6-year-old. Systematic sampling method was used to sample both vegetation and soil. The parameters determined for vegetation in...

Post intervention assessment of long lasting insecticde nets (LLINs) distributed in Kano metropolis, Kano state, Nigeria

Kano state hasdistributed about 4,137,464 Long Lasting Insecticide Treated Net (LLINs) in2009 being one of the main malaria vector control strategy developed by theNigeria Federal Ministry of Health (FMOH) which is in li...

Response surface modeling of natural alizarin production in hairy root cultures of Rubia tinctorum L. upon elicitation with fungal mycelia

The roots of Rubia tinctorum L., the common madder contain natural red dye known as alizarin. In the current study, central composite design of response surface methodology was employed for modeling of fungal elicitor tr...

Download PDF file
  • EP ID EP1063
  • DOI -
  • Views 629
  • Downloads 43

How To Cite

O. J. Akinjogunla*, O. I. Medo, E. P. Philip (2012). Heamolytic activities, deoxyribonuclease production and in-vitro flouroquinolones susceptibility profile of aerobic gram positive cocci associated with acne vulgaris. Scientific Journal of Biological Sciences, 1(2), 52-60. https://europub.co.uk/articles/-A-1063