Response surface modeling of natural alizarin production in hairy root cultures of Rubia tinctorum L. upon elicitation with fungal mycelia
Journal Title: Scientific Journal of Biological Sciences - Year 2016, Vol 5, Issue 11
Abstract
The roots of Rubia tinctorum L., the common madder contain natural red dye known as alizarin. In the current study, central composite design of response surface methodology was employed for modeling of fungal elicitor treatment on natural alizarin production in uniform hairy root cultures of common madder in liquid 1/2 B5 medium. Upon fungal elicitation assay, using two fungal mycelia elicitors (Aspergillus niger and Bipolaris maydis) at three different times (0, 12 and 24h), the production of alizarin was determined. According to the results, after 24h; modeling and optimization conditions, including combination of 2 % of both elicitors for alizarin production equal to 10.0 mg.g-1 DW was evaluated. Optimal process parameters have been determined by using a high desirability value of 1.00 in Design-Expert® software. Our results, altogether, offer a promising method regarding to the improvement of the alizarin production, as a pivotal natural dye in industrial applications.
Authors and Affiliations
Mehdi Ghorbani*| Department of Agronomy and Plant Breeding, University of Tehran, Iran., Mansoor Omidi| Department of Agronomy and Plant Breeding, University of Tehran, Iran., Seyed Ali Peighambari| Department of Agronomy and Plant Breeding, University of Tehran, Iran., Karamatollah Rezaei| Department of Food Science and Technology, University of Tehran, Iran.
Keywords
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- EP ID EP1134
- DOI 10.14196/sjbs.v5i11.2177
- Views 451
- Downloads 30
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