High developmental capability of porcine cloned embryos following trichostatin A-dependent epigenomic transformation during in vitro maturation of oocytes pre-exposed to R-roscovitine
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 4
Abstract
The study was carried out in order to evolve, adopt and optimize the new system for preparation of nuclear recipient cells at different stages preceding the somatic cell nuclear transfer (SCNT) in pigs, including in vitro maturation (IVM) of oocytes. The system was applied to facilitate and accelerate the epigenomic reprogrammability for gene expression of donor cell nuclei that had been transplanted into cytoplasmic microenvironment of recipient oocytes and subsequently underwent the dedifferentiating and re-establishing the totipotent epigenetically conditioned status of their transcriptional activity during the preimplantation development of cloned embryos. The use of trichostatin A (TSA)-mediated epigenetic modulation of in vitro-maturing porcine nuclear recipient oocytes that had been pre-treated with R-roscovitine (R-RSCV) resulted in significantly increased blastocyst formation rate among the cloned embryos compared to the R-RSCV- and TSA-unexposed group (almost 44% vs. 26%).
Authors and Affiliations
Marcin Samiec, Maria Skrzyszowska
Keywords
Related Articles
- EP ID EP70914
- DOI -
- Views 133
- Downloads 0
Hoof size as related to body size in the horse (Equus caballus)
The study was conducted to find out the most reliable parameter of the hoof size in relation to the horse body size, exemplified in mares. The mares of four breeds belonging to different origin types were examined: Pureb...
Selected issues concerning biotechnology of farm animals breeding – a review
Achievements made in the last decades in biotechnology of farm animals breeding allowed to develop this branch of science, improve the animal breeding and also make progress in human and veterinary medicine. The most imp...
Changes in physico-chemical characteristics, somatic cell count and fatty acid profile of Brown Short-haired goat milk during lactation
The main aim of this study was to evaluate the effect of the lactation stage (SL) on the fatty acids (FA) profile of raw milk of Brown Short-haired goats reared under organic regime. An integral part of the study was als...
Effect of management change on selected welfare parameters of cows
The aim of the study was to determine the effect of relocation from a tie-stall barn to the facility with free-stall housing on adaptation of cows measured by behaviours latency and lenghts of the first episodes. Cows w...
Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 days of cold storage
The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar par...