The indel polymorphism in cattle amelogenin gene (AMEL) and its significance for the identification and evolutionary studies
Journal Title: Animal Science Papers and Reports - Year 2010, Vol 28, Issue 4
Abstract
Nucleotide and amino acid sequences of cattle amelogenin, the major protein of forming enamel, were analysed by screening 1547 samples collected from cattle of various breeds. Sequenced was the part of cattle exon six of the AMEL gene (codons from 107 to 186) encompassing so-called hot spot of mutation in the mammals amelogenin gene. The analysis showed the presence of characteristic repeats of 9-nucleotide motifs (triplet PXQ) that makes its structure similar to STR sequences. In Polish Red (PR) cattle (37 cows and 6 bulls) a novel variant of 271 bp was detected on chromosome X. The alignment of sequences obtained showed that a novel variant of the amelogenin gene in PR cattle – AMEL-X(271) – is caused by deletion of one 9-nucleotide motif in position 485-493 (numbering according to Gibson et al. 1991). Irrespectively of the use of length polymorphism in the AMEL gene in early sex determination, the new possibilities in evolutionary and identificatory research are created by analysis of sequence structure. In the amelogenin gene the insertion-deletion of 9-nucleotide motifs can be localized in different sequence positions. As a result, sequences can differ by the number of tandem repeats as well as by the position of indels. Comparison of the AMEL-X gene sequences of Bos/Bison and Bubalus species revealed that distinguishing between the two is feasible not only based on the size of the PCR amplicon but also by the presence of additional 9-nucleotide motif in certain repeat region.
Authors and Affiliations
Beata Prusak, Grzegorz Grzybowski
Keywords
Related Articles
- EP ID EP71499
- DOI -
- Views 127
- Downloads 0
Performance, blood profile, carcass and meat traits and tissue morphology in growing rabbits fed mannanoligosaccharides and zinc-bacitracin continuously or intermittently
The study aimed at evaluation the effect of mannanoligosaccharides (MOS) and zinc-bacitracin (ZnB) administered continuously (+) or intermittently (+/-) upon growing performance, carcass and meat traits, blood profile an...
A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs
The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolat...
The effects of polymorphisms in DGAT1, GH and GHR genes on reproduction and production traits in Jersey cows
The aim of the study was to examine the impact of four single nucleotide polymorphisms in DGAT1, GH and GHR genes on reproduction, production and udder health in Jersey cattle. The study was conducted on 209 cows from th...
Improving nitrogen use efficiency of dairy cows in relation to urea in milk – a review
Dietary protein intake is the most important factor determining milk production, milk composition, milk nitrogen efficiency, urinary nitrogen losses, urea content in milk and consequently, ammonia emissions from dairy co...
cDNA cloning and expression analysis of ATGL gene in four avian species
Adipose triglyceride lipase (ATGL) is a new key triglyceride-specific lipase that participates in the lipolysis in adipose tissue. The full cDNA of ATGL gene in Chinese francolin, pigeon, bengalessfinch and house sparrow...