cDNA cloning and expression analysis of ATGL gene in four avian species
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 3
Abstract
Adipose triglyceride lipase (ATGL) is a new key triglyceride-specific lipase that participates in the lipolysis in adipose tissue. The full cDNA of ATGL gene in Chinese francolin, pigeon, bengalessfinch and house sparrow was cloned to reveal its tissue-specific expression by mRNA real time analysis. The obtained cDNA of chinese francolin ATGL gene (cfATGL) was 1465 bp long, and contained 13 bp 5’-untranslated region (5’UTR) and 1452 bp open reading frame (ORF) encoding a 483-amino acid peptide. All the obtained cDNA of pigeon ATGL gene (pATGL), as well as that of bengaless-finch (bfATGL) and house sparrow (hsATGL) was 1459 bp long, including 13 bp 5’UTR and 1446 bp ORF encoding 481 amino acids. The identities of ATGL gene among these birds occurred no less than 88.4% by homology analysis. As indicated by mRNA real time analysis in Chinese francolin tissues, ATGL gene was predominantly expressed in leg muscle, heart and breast muscles of birds of both sexes. In pigeons, ATGL gene was shown to be predominantly expressed in abdominal fat, subcutaneous fat and breast muscle in males, and in subcutaneous fat, leg muscle, heart and abdominal fat in females. In bengaless-finch, very high ATGL mRNA level was found in subcutaneous, heart, breast muscle, abdominal and leg muscle fat in males, and in breast muscle, leg muscle, abdominal and subcutaneous fat in females. In house sparrow, higher ATGL mRNA level was detected in subcutaneous, breast muscle, leg muscle and abdominal fat in males, and in breast muscle, heart and leg muscle fat in females. In conclusion, the ATGL cDNA of Chinese francolin, pigeon, bengaless-finch and house sparrow was obtained and predominantly expressed in adipose, muscle and heart tissues.
Authors and Affiliations
Xiaomei He, Yongsheng Hu, Han Cai, Ying Li, Meixia Fang, Xinzhheng Jia, Qinghua Nie, Xiquan Zhang
Keywords
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