HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHIRAL SEPARATION OF D,L-PHENYLALANINE AND D,L-TRYPTOPHAN WITH QUATERNARY MOBILE PHASE MIXTURE BY COPPER MIXED CHELATE COMPLEXATION
Journal Title: Analele Universitatii din Bucuresti. Chimie - Year 2010, Vol 19, Issue 2
Abstract
A simple and rapid HPLC enatioseparation of D,L-phenylalanine (Phe) and D,L tryptophan (DL-Trp) was developed. The resolution of enantiomers couples was based in the formation of mixed chelating complexes between copper (II) and L-histidine with quaternary mobile phase. The mobile phase was constituted of buffer ammonium acetate (pH=7), acetonitrile, isopentyl alcohol and isopropyl alcohol. An excellent separation of D, L couples was obtained with good selectivity and in no more than 15 min.
Authors and Affiliations
B. Saïdat, Fouzia Boudah, M. H. Guermouche
Keywords
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