Histological And Histochemical Study of Peritoneal Mast Cells of Albino Rat Using Two Types of Fixatives.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 4
Abstract
Introduction: Mast cells have a widespread distribution and are found predominantly at the interface between the host and external environment. Aim and Objectives: The main purpose of the present work is to histologically and histochemically study the properties of Peritoneal Mast cells of Albino Rat. Materials and Methods: The stretch preparation of tissues were taken from albino rat and fixed in two types of fixatives; Formal Alcohol and Neutral buffered formalin.Then subjected to various histochemical stains. Results: Peritoneal mast cells were found in good amount in Albino rat they are better stained in toulidine blue at ph 4.4 in neutral buffered formalin than in formal alcohol while with toulidine blue at ph 2 the reactions are similar with both the fixatives.Heparin Trisulphate was substantial whereas Heparin monosulphate and Neutral mucopolysaccaride were presentl in small amount in mast cells of albino rat. Conclusion: Heparin Trisulphate is an active form of Heparin and is the main constituent of the peritoneal mast cell granules of Albino rat.
Authors and Affiliations
Dr Ashok Kumar Dubey, Dr Alka Rashmi Nag
Keywords
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