Histological Assessment of the Testes and Serum Testosterone of Adult Male Albino Wister Rats Following Oral Administration of Ground Nutmeg Seed
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2018, Vol 4, Issue 10
Abstract
This research was designed to assess the effects of oral administration of varying doses of ground Nutmeg seed on the histomorphology of the testes and the hormonal profile of thirty adult male albino wister rats weighing between 140 g and 250 g. The rats were divided into six equal groups, namely A,B,C,D,E and F. Group A served as the control and were administered only water and 100 g of rat feed; while groups B,C,D,E and F, the treatment groups, were administered varying doses (1 g, 5 g, 10 g, 15 g and 20 g/kg body weight, respectively) of the ground Nutmeg seed, mixed with 100 g of rat feed, respectively, for 26 days. The weight measurement was done every seven days. The testes were harvested immediately after sacrifice, fixed in 10 % buffered formalin, and was later made to undergo Hematoxylin and Eosin (H&E) staining method. Blood samples were collected via cardiac puncture for serum testosterone analysis. Data were expressed as Mean + standard error of the Mean and subjected to one way analysis of variance. Significance different between mean was assessed by student-New-Man-Keuls post hoc test. 95 % level of significance (P = 0.05) was used for statistical analysis and Microsoft Excel 2010 package was used for graph and error bars. The histopathological results revealed no structural defect on the testes of rats administered 1 g and 5 g of ground nutmeg. There was dose dependent alteration of the histomorphology of the testes of rats given 10 g and above. There was also persistent elevation in serum testosterone level in treatment groups B to F, compared to the control Group A. Hence, consumption of high dose of nutmeg is toxic to the testes.
Authors and Affiliations
Christopher Chiedozie Mbadugha, Ebenezer Reuben Oke, Leko Bankole Japhet
Keywords
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