HIV-1 subtypes and drug-resistance mutation sites in HIV/AIDS patients with antiretroviral-therapy failure
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 3
Abstract
ObjectiveTo analyze the characteristics of HIV-1 subtypes and drug-resistance mutation sites among HIV-infected patients who received high-efficiency antiretroviral therapy but failed.MethodsA total of 130 plasma samples were collected from the patients who received antiviral treatment for 6 months in Taizhou City of Zhejiang Province in 2021 but failed the treatment and the viral load was â¥1 000 copies·mL-1. Nucleic acid in the samples was extractedï¼ and the pol gene was amplified by nested reverse transcription PCR. After next-generation sequencingï¼ online tools were used to compare and analyze the subtypes and drug-resistant mutation sites.ResultsA total of 110 samples were successfully sequenced. The main HIV-1 subtype was CRF01_AEï¼ accounting for 42.72% ï¼47 casesï¼ï¼ followed by CRF07_BCï¼ 35.45% ï¼39 casesï¼ï¼ CRF08_BCï¼ 10.00% ï¼11 casesï¼ï¼ CRF85_BC ï¼ 8.18% ï¼9ï¼ï¼ and a small number of B subtypeï¼ 1.81% ï¼2 casesï¼ and C subtypeï¼ 1.81% ï¼2 casesï¼. The online tool comparison showed that there were 67 cases with mutations of drug-resistance sites and 61 cases with drug-resistance. The mutation sites were mainly M184Vï¼ K103Nï¼ K65R and V181Cï¼ and the mutation rates were 20.00% ï¼22 casesï¼ï¼ 10.91% ï¼12 casesï¼ï¼ 8.18% ï¼9 casesï¼ and 8.18% ï¼9 casesï¼ï¼ respectively. These mutation sites caused different degrees of resistance to nucleoside reverse transcriptase inhibitors ï¼NRTIï¼ï¼ non- nucleoside reverse transcriptase inhibitors ï¼NNRTIï¼ and protease inhibitors ï¼PIï¼ï¼ including 45 cases of NRTIï¼ 61 cases of NNRTI and 2 cases of PI resistance.ConclusionThe HIV infected people who fail the treatment in Taizhou are mainly with the subtypes CRF01_AE and CRF07_BC. The rate of drug-resistance mutation is at a moderate levelï¼ mainly due to the mutation of NRTI and NNRTI drug-resistance sitesï¼ and a small number of PI drug-resistance sites. Thereforeï¼ the antiviral treatment plan for HIV infected people should be reasonably adjustedï¼ and the detection of drug-resistance mutation sites should be strengthened to avoid the generation of transmissible drug-resistance strains.
Authors and Affiliations
LIN Junxiao,WANG Hongzhu,LI Congcong,SHENG Ying,LI Guixia,
Keywords
Related Articles
- EP ID EP742089
- DOI -
- Views 34
- Downloads 0
Analysis of the punishment on unlicensed medical practice in Jinshan District of Shanghai
ObjectiveThis paper analyzed the punishment situation of unlicensed medical practice in recent 7 years in Jinshan District of Shanghaiï¼ then suggested the countermeasures to prevent and combat unlicensed medical practi...
Study on the progress of HIV typing methods
As a retrovirus with high recombination and mutation rateï¼ HIV targets CD4+ T lymphocytesï¼ causing damage to the body's immune system and eventually leading to severe immune function defects. Different subtypes of th...
Analysis of enterovirus serotype results in Dapeng New District， Shenzhen from 2016 to 2022
Objective To provide a basis for human enteroviruses prevention and control by monitoring the enterovirus （EV） and its main virus types.Methods Samples of hand-foot-and-mouth disease， herpetic angina and fever clinic...
Research progress in prevention and disease management of osteoporotic fractures
Osteoporotic fracture is the most major complication of osteoporosis. It is a significant global public health problem which continues to bring serious family and social burden. In order to improve the prevention and tre...
Research progress on reproductive and developmental toxicity of e-cigarettes
E-cigarettes have gained considerable popularity worldwide recentlyï¼ which have also drawn the attention of adolescents and pregnant women. Howeverï¼ recent studies have uncovered the negative effects of e-cigarettes...