How would Dexmedetomidine have Protective Effects on the Neurological Scores, Brain Edema and Brain-Blood Barrier Post to Serious Traumatic Brain Damage in Male Rats? (The Possible Role of MMP-9)
Journal Title: The 1st Annual Meeting of Georgian Center for Neuroscience Research - Year 2020, Vol 2, Issue 20
Abstract
Introduction: Dexmedetomidine DEX is known as a α2-adrenergic receptor agonist. It is used as a short-acting analgesic drug. In this study, we investigated the effects of neural protection of the DEX after induction of cerebral inflammation in rats. Materials and methods: The male Albino wistar rats received different doses of DEX (15, 30, 60 mg/kg, i.p.). All rats were intubated before TBI. In the TBI groups, diffuse TBI was induced by Marmarou method using a TBI induction device. The severe TBI was induced using a weight 300 gr. In the same groups, all stages of induction of TBI were performed except dropping weight on the head. The disruption of Blood brain- barrier (BBB) was evaluated 6 h post- TBI. The neurologic score (VCS) and brain water content, the beam-walk –balance task (WB) were determined before trauma, on trauma time(D0), and day 1 (D1) and Day 2 (D2) and Day 3 (D3) After TBI. after 72 hour CSF samples are collected from cisterna magna and then analysis mmp9 in CSF with Elisa assay. Results: Our results showed that traumatic brain injury led to significant brain edema and disrupt of blood brain- barrier and neurological defect and vestibular-motor dysfunction in the rat brain and increase mmp9 in CSF serum. DEX (15,30mg/kg) could attenuated brain edema, improved BBB, vestibular-motor dysfunction and decreased mmp9 in compare with TBI control group (P<0.001) but in 30mg/kg dose results were better and didn't have neuroprotective effect in 60mg/kg dose. Discussion: These findings showed that DEX has a prominent role in TBI outcome’s and perhaps protect neurons through modulating inflammatory and antioxidant pathways by inhibition of increasing intracellular calcium and decreasing mmp9 factor. By adding α2-adrenergic antagonist, neuroprotective effect of Dex has been disappeared. it indicates that a part of neuroprotective effect of DEX is through α2-adrenergic receptor.
Authors and Affiliations
Asal Safarbalou, Negar Mehrbakhsh, Mohaddese Shafiee Pour, Ali Siahposht Khachaki
Keywords
Related Articles
- EP ID EP677929
- DOI 10.29088/GCNR-2020.20
- Views 169
- Downloads 0
Effect of Clot Lytic of Fig on Brain Stroke
Introduction: The fig (Ficus carica L.), one of the most important fruit species which grows in tropical and subtropical regions of the world. In the native system of medicine different sector (fruit, root and leaves) a...
Investigating the Relationship Between Impulsivity and Deficit in Executive Functions of Brain in Patients with Substance Use Disorder
Introduction: In recent years, research into the biological foundations of addiction has attracted the attention of many researchers. Researchers found that substance use is the result of greater activity of the brain b...
Nigella Sativa Oil Enhanced Motor Coordination in Phenol-Induced BALB/c Mice Models of Essential Tremor
Background: Essential tremor is a neuronal disorder characterized by uncontrollable tremor of different body parts. Nigella sativa is an established therapeutic plant in various ailments including neurological disorders...
The Nucleus Accumbens Neurons Firing Pattern during the Extinction and Reinstatement of Morphine-Induced Conditioned Place Preference in Rats: Electrophysiological Roles of Glutamate Receptors
Background and Aim: The mesocorticolimbic dopaminergic system projecting from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) is necessary for the initiation of opioid compulsive usage and reward-associa...
QEEG and Monitoring of Anticonvulsive Treatment in Epileptic Children
Background: The study aimed to reveal EEG baseline patter for optimization of anti-epileptic treatment in individual epileptic patients. Such type of investigation is very important, because characteristics of baseline...