HPLC/MS/MS Study of Phenolic Compounds of Leucaena leucocephala Legumes Monitored with Their in vitro Antihyperglycemic Activity
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 17, Issue 4
Abstract
High pressure liquid chromatography coupled with mass spectrometry (HPLC/MS/MS) is considered as one of most the sensitive modern techniques used for determination of phenolic compounds, hence in this research it was utilized for characterization of a variety of phenolic compounds in Leucaena leucocephala legumes growing in Egypt. HPLC gradient elution analysis using water and acetonitrile, both containing 0.1% formic acid was carried out for standard solutions of the available phenolic and flavonoid compounds as well as the investigated fraction in the negative ion mode using MS/MS product ion scans where the deprotonated molecules [M‒H]- were observed for all the studied compounds; for flavonol and flavone glycosides, the spectra exhibit both the deprotonated molecule [M‒H]‒ of the glycoside and the ion corresponding to the deprotonated aglycone [A‒H]–, the latter ion is formed by loss of the sugar residue from the glycosides; different fragmentation pattern was observed for flavone-C-glycosides which involved additional fragmentation in the sugar part while for methyl ether aglycones, formation of [M‒CH3]‒ • was observed. The gained results revealed the elution of eleven phenolic compounds from the ethyl acetate fraction, namely; isovanillin, gallic acid, caffeic acid, apigenin-8-C-glucoside (vitexin), quercetin 3-O-galactoside (hyperoside), luteolin-7-O-glucoside, quercetin, isorhamnetin, luteolin 6-methyl ether (neptein), kaempferol 3,7-dimethyl ether and 5,7,3',4'-dihydroquercetin tetramethyl ether (taxifolin 5,7,3',4'- tetramethyl ether) successively. The methanol extract and different fractions of Leucaena leucocephala legumes were subjected to in vitro evaluation of their α-glucosidase inhibitory activity at different dose levels compared to the reference standard drug “Acarbose”, the gained results revealed that methanol extract and ethyl acetate fraction exhibited significant percent inhibitory activity at dose level of 2.5 mgml-1 (71.55±0.009%, IC50 0.210±0.003 mg ml-1 & 65.44±0.005%, IC50 0.270±0.042 mg ml-1) respectively compared to Acarbose (55.45±0.051%, IC50 0.650±0.051 mg ml-1) which affords both as effective natural antihyperglycemic drugs.
Authors and Affiliations
Nevein M. Abdelhady, Gamil M. Abdallah
Keywords
Related Articles
- EP ID EP349615
- DOI 10.9734/EJMP/2016/31403
- Views 103
- Downloads 0
Evaluation of Ex-vivo Anti-arthritic, Anti-inflammatory, Anti-cancerous and Thrombolytic Activities of Mussaenda roxburghii Leaf
Aim: The current study evaluates the anti-arthritic, anti-inflammatory, anti-cancerous and thrombolytic activities of methanolic extract of Mussaenda roxburghii leaf. Place and Duration of study: This study was conducted...
In vivo Toxicological Assessment of the Aqueous Extracts of the Leaves of Carissa edulis (Apocynaceae) in Wistar Rats
Aim: To screen the acute and 28-days subchronic toxicity of the leaves aqueous extract of Carissa edulis (C. edulis). Design: Experimental and observational study. Place and Duration: Faculty of health sciences of Cotono...
In vivo Anticoccidial and Antioxidant Activities of Psidium guajava Methanol Extract
Background: Coccidioses are the major parasitic diseases in poultry and other domestic animals including the domestic rabbit (Oryctolagus cuniculus).The aim of this study was to evaluate in vivo anticoccidial and antioxi...
Evaluation of Hepatoprotective Effect of Methanol Extract of Solanum melongena on Carbon Tetrachloride Induced Hepatotoxic Rats
Aim: To evaluate the effect of methanol extract of Solanum melongena on carbon tetrachloride- induced liver damage in albino rats. Methodology: The qualitative and quantitative phytochemicals screening of the plant extr...
Evaluation of Molluscicidal Activity of Croton microstachyus and Cissus quadrangularis against Biomphalaria and Bulinus Species of Snails
Aim: The study was carried out to investigate the molluscicidal activities of different solvent extracts of two Eritrean local plants, Croton macrostachyus and Cissus quadrangularis identified using ethno-botanical, phar...