Hypericum Humifusum Leaves Attenuates Hepatic Ischemia-Reperfusion Injury in a Rat Model
Journal Title: Annals of Hepatology - Year 2018, Vol 17, Issue 1
Abstract
Introduction. Effective prevention strategies require specific actions during the different phases of ischemia-reperfusion (I-R) injury. The objective of our study is to evaluate the effect of aqueous extract of Hypericum humifusum leaves (HHL) on liver I-R model in Rat. Material and methods. Animals were subjected to 90 min of hepatic ischemia followed by reperfusion (120 min). HHL extract (25 mg/mL/kg) was injected 15 min before reperfusion. To evaluate the effect of HHL extract on I-R, we have monitored transaminases levels, Malondialdehyde (MDA) concentration, histological lesions (apoptosis and necrosis) and compared the results to a reference oxidant vitamin E. Results. The determination of total phenol extracts of HHL was 59.91 ± 0.35 mg of Gallic Acid/g dry plant material with higher antioxidant activity (91.73% ± 1.67) compared to vitamin E (87.42%). Using aqueous extract of HHL, we noted a significant decrease of AST and ALT [1129 UI (585/1995) and 768 UI (335/1375)] compared to no-treated group [5,585.5 UI (5,035/12,070) and 8,099.5 UI (5,040/12,326)] as a decrease in MDA content [85.7% protection (50.9/91.5)]. HHL extract reduce the damage induced by I-R of 48.7% (27/48.7) and 96.1% (95.7/96.5) for necrosis and apoptosis lesions respectively. Conclusion. HHL aqueous extract have potential to protect liver from the damage effect induced by I-R better than vitamin E solution.
Authors and Affiliations
Henda Ferchichi, Issam Salouage, Sarra Bacha, Nadia Kourda, Emna Gaies, Anis Klouz, Sameh Trabelsi
Keywords
Related Articles
- EP ID EP247967
- DOI 10.5604/01.3001.0010.7546
- Views 78
- Downloads 0
Severe aplastic anemia-paroxysmal nocturnal hemoglobinuria syndrome in a patient with non-A-E hepatitis virus infection
Hepatoprotective effect of stem of Musa sapientum Linn in rats intoxicated with carbon tetrachloride
Methods. The study was designed to evaluate the hepatoprotective activity of aqueous extract of central stem of Musa sapientum (AqMS) against carbon tetrachloride induced hepatotoxicity in rats. Animals were divided into...
High ghrelin and obestatin levels and low risk of developing fatty liver
Aim. Obesity and insulin resistance are associated with nonalcoholic fatty liver disease (NAFLD). It was recently reported that the ratio between levels of ghrelin and obestatin is also associated with obesity and insuli...
Focal nodular hyperplasia vs. hemangioma:Computed tomography and magnetic resonance imaging findings
Focal nodular hyperplasia (FNH) and hemangioma are benign and generally asymptomatic hepatic tumors. With distinctive imaging findings on dynamic computed tomography (CT) and magnetic resonance imaging (MRI), differentia...
Mycobacterium avium complex in the liver