HYPOGLYCEMIC, CELL MEMBRANE STABILITY, THROMBOLYTIC, TOTAL PHENOLIC CONTENT AND FREE RADICAL SCAVENGING ACTIVITIES OF METHANOLIC EXTRACT OF ZIZIPHUS MAURITIANA FRUITS (RHAMNACEAE)
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 3
Abstract
The purpose of the present study was to evaluate in vitro free radical scavenging activity and total phenolic content; hypoglycemic activity, cell membrane stabilizing activity and thrombolytic activity in mice model of methanolic crude extract of Ziziphus mauritiana fruits. The coarse powder of dried fruits was soaked with methanol for several days with occasional stirring. After that it was extracted at room temperature and dried extract was partitioned into pet ether, chloroform, carbontetrachloride and aqueous soluble fraction. Among all the fractions, aqueous soluble fraction revealed highest free radical scavenging activity having IC50 value 26.77 µg/ml flowed by chloroform soluble fraction of 43.46 µg/ml with respect to BHT having IC50 value 24 µg/ml. The amount of total phenolic content of different extractives ranged from 29.30 mg to 52.18 mg of GAE / g of extractives of Z. mauritiana and pet ether soluble fraction showed the highest phenolic content 52.18 mg of GAE / g of extractives. Crude extract at a dose of 400 mg/kg body weight moderately lowered the blood glucose level after 1st, 2nd and 3rd hour having 37.81, 43.65 and 44.4 percent of fall of blood glucose level and exhibited highest thrombolytic activity 50.22 % clot lysis in mice model. In hypotonic solution induced condition carbontetrachloride and pet-ether soluble fraction inhibited 54.55 % and 52.8 % haemolysis of RBC with respect to 69.9 % revealed by acetyl salicylic acid (0.10 mg/mL); heat induced condition crude extract, pet ether and aqueous soluble faction revealed moderate inhibition about 29.39, 30.90 % and 28.19 % compared with 40.20 % inhibition by acetyl salicylic acid in heat induced condition.
Authors and Affiliations
Hamiduzzaman Md. , Uttom Kumar, Sultana Mannan
Keywords
Related Articles
- EP ID EP126255
- DOI 10.7897/2230-8407.050331
- Views 91
- Downloads 0
A CRITICAL REVIEW ON KAKANDOLA: AN AYURVEDIC HERB
Kakandola, since ages has been used on the name of the herb named kapikachhu, for its medicinal properties. It is said to have the gunas like that of masha (Phaseolus mungo Linn.) and is used for its vrishya karma. In ma...
GREEN CHEMISTRY POTENTIAL FOR PAST, PRESENT AND FUTURE PERSPECTIVES
Chemistry brought about medical revolution till about the middle of twentieth century in which drugs and antibiotics were discovered. The world’s food supply also increased enormously due to the discovery of hybrid varie...
TASTE MASKING IN PHARMACEUTICAL: AN UPDATE
Taste is an important factor in the development of dosage form. Nevertheless it is that arena of product development that has been overlooked and undermined for its importance. The problem of bitter and obnoxious taste o...
SCREENING OF HERBAL LEAVES EXTRACT OF BOUGAINVILLEA GLABRA FOR DIABETIC NEPHROPATHY IN RATS
The main objective of this study was to investigate hypoglycemic effects of Bougainvillea glabra Leaves extract in Streptozotocin (STZ) induced hyperglycemic rats. The secondary objective of the study was to study effect...
CHEWABLE LOZENGE FORMULATION- A REVIEW
Development of lozenges dated back to 20thcentury and is still remain popular among the consumer and hence it has continued commercial production. Lozenges are palatable solid unit dosage form administrated in the oral c...