Identification and Enumeration of Bacterial Species From Rice Rhizosphere to Determine the Ratio of Nitrogen Fixers on Agricultural Aspect
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
Soil sample was collected from agricultural land of Chuadanga district, Bangladesh for Microbial Characterization and Analysis of N2-Fixing bacterial species. The process was initiated with rice rhizosphere during seedling (after 30 days) of rice (BR 28, Oryza sativa L.). N-fertilizers mixing ration at that soil was 60kgha-1yr.-1. The soil was usually cultivated by Aus, Aman and Boro crops, as well as Rabi crops in winter. 1500 grams of crushed wet soil samples were collected. Drying process was initiated under 65°C for four days. 1227 grams samples were found after drying process which was then separated into 4 equal parts for post processing. Total 188 isolates were found in which only 65 were nitrogen fixer, in a ratio of 34.58%. Isolated nitrogen fixer were observed physiologically and biochemically and tested with a variety of enzymatic reaction, four species were found based on study criteria- Enterobacter spp., for strain-1, Klebsiella spp. for strain-2, Bacillus spp. for strain- 3 and Azospirillum spp. for strain-4.
Authors and Affiliations
Sazin Islam, Md. Masiuzzaman, Toriqul Islam1, Muhammad Arif Iqbal1, Md. Jahid Al Jakaria, Md. Mahabub Hasan, Md. Mohbub Alam
Keywords
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- EP ID EP387172
- DOI 10.9790/3008-1106066265
- Views 85
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