Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 2
Abstract
β-D-glucosidase (βG) gene from [i]Oenococcus oeni [/i]SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. Results showed that βG gene of the two strains was in high homology (&;;gt; 99%) to reported βG gene, confirming both strains possess βG activity on molecular. Intracellular βG of SD-2a is mainly soluble protein, existing most in cytoplasm and some in periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in cytoplasmic membrane. This study provides basic information for further study on the metabolic mechanism of βG from [i]O. oeni[/i] SD-2a and 31MBR.
Authors and Affiliations
Yahui Li, Yanhong Ma, Kaihong Huang, Hongzhi Zhang
Keywords
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