IDENTIFICATION OF ARYLTETRALIN LIGNANS FROM PODOPHYLLUM HEXANDRUM USING HYPHENATED TECHNIQUES
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 1
Abstract
In the present study, with the appliance of hyphenated techniques involving high performance liquid chromatography with diode array detection directly coupled with 1H nuclear magnetic resonance spectroscopy (LC-NMR) and electrospray ionization mass spectrometry (LC-ESI-MS), four known aryltetrahydronephthalene lignans (podophyllotoxin 4-O-glucopyranoside, 4’-demethylpodophyllotoxin, podophyllotxin and dehydropodophyllotoxin) have been well characterized from methanol extract of rhizomes of Podophyllum hexandrum Royle without involving the time consuming steps of isolation and purification of individual constituents of the extract. Also, a simple, sensitive and reproducible LC-MS method has been developed for the quantitative analysis of podophyllotoxin in crude methanolic extract from different parts of Podophyllum hexandrum Royle. The present work adds up to the existing significance of hyphenated techniques combining chromatographic technologies with spectroscopic techniques for both qualitative and quantitative analysis of natural product extracts or fractions without any need of reference standards for the markers.
Authors and Affiliations
Ekta Sharma, Bhupinder Singh Arora
Keywords
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