Identification of Biochemical Variation in Alternaria Isolates of Onion Plant
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 7
Abstract
Biochemical variability among the 15 isolates of Alternaria isolates from different locations of West Bengal was investigated in respect of isozyme to observe the polymorphism among the isolates. Electrophoretic separation of the extracts was carried out on native PAGE following the procedure and gels were stained for different enzymes. The activity of α- esterase produced distinctive bands that were dark brown, whereas the activity of β- esterase produced bands that were dark pink so that α- esterase and β- esterase were scored very easily. Two separate runs were conducted to determine reproducibility of the bands and to calculate the relative mobility (Rm). Values of each of the isozyme based on the presence or absence matrix of the bands of each isolate. Positive activity was observed for both α- and β- esterase. α- esterase enzyme showed the highest enzyme activity in terms of maximum numbers of banding loci among the two isozymes tested.
Authors and Affiliations
Srujani Behera, Subrata Dutta, Srikanta Das
Keywords
Related Articles
- EP ID EP580921
- DOI 10.20546/ijcmas.2018.707.004
- Views 73
- Downloads 0
Antifungal Effect of Chitosans and Chito-oligosaccharides against Early Blight of Tomato
The present investigation was to determine the antifungal ativity of different chitosans and chito-oligosaccharides against Alternaria solani. Out of four chitosans tested, chitosan DA10 and 134 was recorded as 53.44 and...
Role of Male Sterility in Vegetable Hybrid Seed Production
Hybrid varieties play a vital role in increasing vegetable production due to their high yield potential, early maturing, superior quality, disease and pest resistance attributes. Male sterility in vegetables is commercia...
Standardization of Tinting Techniques in China aster cv. Local White
Value addition in floriculture increases the economic value and consumer appeal of any floral commodity. The profitability of a commodity is increased when the raw material is converted into a value added product which c...
Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia
Aquaculture is one of the fastest growing food production sectors globally. Tilapia is the second widely farmed fish species in the global fish production. Enterococcus sp. is one of the leading causes of nosocomial infe...
Microbiological Analysis and Nutritional Evaluation of Cottage Cheese Produced with Kiwifruit Enzyme
Cottage cheese (soft cheese) is an excellent source of protein, fats and minerals such as calcium, iron and phosphorus, vitamins and essential amino acids, thus making it an important food in the diet of both old and you...