IDENTIFICATION OF CLINICALLY RELEVANT MYCOBACTERIUM OTHER THAN TUBERCULOSIS (MOTT) SPECIES BY REAL-TIME PCR COUPLED WITH A HIGH-RESOLUTION MELTING SYSTEM
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 6
Abstract
A real-time PCR coupled with a high-resolution melting curve assay for identifying clinically relevant MOTT species was developed and evaluated using 6 reference strains and 186 Culture isolates. Concordant results were obtained for all 186 isolates of nontuberculous mycobacteria. Our results showed that this real-time PCR -HRM assay is an effective tool for the mycobacterial identification from cultures isolates.
Authors and Affiliations
Prem Raj Singh| Auroprobe Laboratories NH-58 Muradnagar, Ghaziabad (U.P), India., Devender Singh Gill| Auroprobe Laboratories NH-58 Muradnagar, Ghaziabad (U.P), India., Preeti Caesar| Auroprobe Laboratories NH-58 Muradnagar, Ghaziabad (U.P), India., Veena Sharma*| Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India.
Keywords
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