Identification of Malassezia species using PCR-RFLP molecular method in the patients with pityriasis versicolor
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2017, Vol 22, Issue 89
Abstract
Background and Aim: Malassezia yeast can become pathogenic under certain conditions in humans. Malassezia has various species which can cause a number of skin diseases, such as pityriasis versicolor (tinea versicolor), seborrheic dermatitis and folliculitis and even systemic infections. In the present study, Malassezia species isolated from the patients with pityriasis versicolor were identified by PCR-PFLP molecular method. Material and Methods: In this study, the scraping specimens of the trunk and scalp of the patients with pityriasis versicolor were cultured on Dixon agar medium. Finally, one-hundred Malassezia colonies were obtained. The genomic DNA was extracted by phenol–chloroform method and then was studied by use of PCR-PFLP molecular method. D1-D2 segment in the area of 26srDNA of ITS gene was proliferated by specific primers and then the PCR products were exposed to CfoI restrictive enzyme. Results: Among 100 Malassezia colonies, the most common Malassezia species were; M. globosa (44%), M. globosa/M. restricta (27%), M. restricta (11%), M. sympodialis (7%), M. sympodialis/M. restricta (4%), M. globosa/M. restricta/M. furfur (1%), M. sympodialis/M. restricta/ M. globosa (1%) and unknown species (5%). Conclusion: The dominant species isolated from patients with pityriasis versicolor were M. globosa, M. restricta and M. sympodialis, respectively. Thirty-three percent of the specimens had more than one Malassezia species. Therefore, rapid PCR-RFLP method is recommended for identification of Malassezia species in epidemiological studies and production of more effective medicines.
Authors and Affiliations
PhD M Hosseini Bafghi, PhD N. A Mozafari, PhD A. M Fata, PhD A Naseri, PhD H Zarrinfar
Keywords
Related Articles
- EP ID EP282899
- DOI -
- Views 93
- Downloads 0
Evaluation of heavy metals in tobacco and hookah water used in coffee houses in Sanandaj city in 2017
Background and Aim: Tobacco use in various ways such as cigarettes, hookahs, and nas is one of the most important and preventable factors in illness, disability and premature death in the world. Considering the importanc...
Protective effects of hydro-alcoholic extract of Allium cepa on biochemical and morphometric parameters of mice kidneys exposed to formaldehyde
Background and Aim: Formaldehyde is an air contaminant which has wide applications in industry and medicine and its destructive effects have been reported on the body systems. The aim of this study was to investigate th...
Frequency of human papilloma virus genotypes Among woman with genitalia lesion , Sanandaj ,Iran
Background and Aim: Genital wart is the most common sexually transmitted infection and its role in cervical cancer has been confirmed. The aim of this study was to determine the presence of human papillomavirus (HPV) and...
Evaluation of astaxanthin effects on the survival and proliferation of human adipose derived stem cells
Background and Aim: In order to enhance in vivo stem cell viability and considering similar anti-inflammatory and anti-apoptotic effects of human adipose derived stem cells (hADSCs) and astaxanthin (AST) and their abilit...
Biochemical and histopathological study on preventive effects of Punica granatum L. extract on fatty liver disease in the rats receiving high fat diet.
Background and Aim: Non-alcoholic fatty liver disease (NAFLD) is recognized as a metabolic liver disease in the world. The purpose of this study was to investigate the preventive effects of Punica granatum extract on fat...