Identification of Metabolites through GC/LC–MS Processed Data using Different Reference Libraries and Their Comparison
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2016, Vol 6, Issue 6
Abstract
Much significant advancement has been reported in the last few years in the field of metabolomics studies. The high-end computer applications are already contributing to the research and analysis in the field of life sciences. There are many hardware and softwares available, which can be used with various biomolecular separation and analysis instruments like chromatography, mass spectroscopy (MS), NMR, etc. The metabolite identification is the crucial part of the metabolomics study. The biosample collected from any resource need to be analysed from GC/LC–MS or NMR-type instrumentation to precisely identify the compounds present in the sample qualitatively and quantitatively. There are many tools and databases already available which can be used for the pre-processing, processing and analysis of raw data generated from these instruments. Various reference libraries are also available, which can be used for the identification of metabolites present in the sample after the processing of raw data. In this study, we have reviewed and compared different libraries and tools available for the metabolite identification from GC/ LC–MS data.
Authors and Affiliations
Priya Ranjan Kumar, Sarika Srivastava, Santosh Kumar Mishra
Scar Endometriosis: A Case Report
Endometriosis is defined as functioning endometrial glands and stroma outside the uterus. Pelvic endometriosis is more common than extrapelvic sites. Among extrapelvic sites, scar endometriosis is a very rare entity and...
Oral Misoprostol Versus Intravenous Methylergometrine for the Prevention of Postpartum Haemorrhage
Background Post partum haemorrhage is one of the most common causes of maternal mortality. Uterine atonicity, trauma to the genital tract, retained product of placenta are common causes of postpartum haemorrhage (PPH), o...
Biofilm Formation and Methicillin Resistance among the Staphylococcus aureus Causing Burn Wound Infections in a Tertiary Care Hospital: A Comparative Study of the Antibiotic Susceptibility Pattern Between Biofilm Producers and Non Biofilm Producers
Background Staphylococcus aureus is one of the common bacteria implicated in burn wound infections that possess the ability to form biofilms. Aim The present study aims to isolate S. aureus to identify the Methicillin re...
Quality-Adjusted Life Years (QALYs) of Tuberculosis Patients in the Intensive and Continuous Phase in a Private Hospital of Yogyakarta, Indonesia
Context Tuberculosis is a disease with low quality of life (QoL) and quality-adjusted life years (QALYs) due to illness, impairment of health conditions and death. QALYs combine the QoL as well as quantity of life with v...
In vitro Antifungal Activity of Pluronic-based Thermo-Sensitive Itraconazole Gels for Vaginal Administration
Vulvovaginal candidiasis (VVC) is a common genital disease disturbing women. ITZ is widely used for the treatment of VVC by oral administration in clinic. Due to its irregular absorption and systematic side effects, loca...