Identification of rod-shaped Gram-negative bacilli of Enterobacteriaceae family by MALDI-TOF mass spectrometry
Journal Title: Postępy Nauk Medycznych - Year 2015, Vol 28, Issue 4
Abstract
Introduction. Matrix assisted laser desorption ionization-time of flight mass spectrometry is a new rapid method for identification of microorganisms isolated from clinical specimens.Aim. The aim of the study was to compare the results of identification Enterobacteriaceae strains obtained by using MALDI Biotyper and Vitek 2.Material and methods. Identification of 313 Enterobacteriaceae isolates obtained from clinical specimens was performed simultaneously by using MALDI Biotyper and Vitek 2.Results. In the case of 84.98% of the strains (n = 266) the same effectiveness of identification to the species level was obtained using both analyzers. 13.42% strains (n = 42) have been equally identified only to the genus level by both analyzers. Only in the case of 4 strains (1.28%) the results were divergent.Conclusions. MALDI TOF-MS technology provides an excellent tool for identification of bacteria from clinical specimens. It allows obtaining reliable results within time unattainable for biochemical methods of microorganisms identification.
Authors and Affiliations
Robert Kuthan, Łukasz Chabros, Anna Sawicka-Grzelak, Grażyna Młynarczyk
Keywords
Related Articles
- EP ID EP55035
- DOI -
- Views 186
- Downloads 0
Choroba alzheimera w systemie rodzinnym z perspektywy psychologicznej.
Artykuł jest rodzajem podsumowania pracy z prowadzonej przez autorki grupy wsparcia. W jej skład weszli opiekunowie pacjentów ze zdiagnozowaną chorobą Alzheimera (Alzheimer disease - AD). W doniesieniu przedstawiono sytu...
An analysis of discrepancies between test results of the selected protein parameters and clonal bone marrow plasma cells in an assessment of treatment response in a group of patients with multiple myeloma (MM) prior to an autologous hematopoietic stem cell transplantation (AHSCT)
Introduction. Multiple myeloma (MM) is an incurable, B-cell malignancy. Modern diagnostics and disease monitoring include the assessment of serum and urine protein as well as bone marrow examination. The discrepancies of...
Carotid artery stenting – 15 years of single centre experience
<b>Introduction. </b>About 20% of ischemic strokes are caused by extracranial carotid artery stenosis, mainly due to atherosclerosis. Patients with hemodynamically important carotid stenoses n...
Alergiczne zapalenie pęcherzyków płucnych
Alergiczne zapalenie pęcherzyków płucnych (AZPP) jest chorobą zapalną miąższu płuc i oskrzelików, związaną z powtarzanym narażeniem na wziewne antygeny środowiskowe, którymi są białka bakterii, grzybów, roślin i zwierząt...
Is there really an epidemic of autism?
Autistic disorder, pervasive developmental disorder-not otherwise specified and Asperger syndrome belong to a group of disorders called autism spectrum disorders (ASDs). In the past 25 years, an increase in the prevalenc...