Identification of rod-shaped Gram-negative bacilli of Enterobacteriaceae family by MALDI-TOF mass spectrometry
Journal Title: Postępy Nauk Medycznych - Year 2015, Vol 28, Issue 4
Abstract
Introduction. Matrix assisted laser desorption ionization-time of flight mass spectrometry is a new rapid method for identification of microorganisms isolated from clinical specimens.Aim. The aim of the study was to compare the results of identification Enterobacteriaceae strains obtained by using MALDI Biotyper and Vitek 2.Material and methods. Identification of 313 Enterobacteriaceae isolates obtained from clinical specimens was performed simultaneously by using MALDI Biotyper and Vitek 2.Results. In the case of 84.98% of the strains (n = 266) the same effectiveness of identification to the species level was obtained using both analyzers. 13.42% strains (n = 42) have been equally identified only to the genus level by both analyzers. Only in the case of 4 strains (1.28%) the results were divergent.Conclusions. MALDI TOF-MS technology provides an excellent tool for identification of bacteria from clinical specimens. It allows obtaining reliable results within time unattainable for biochemical methods of microorganisms identification.
Authors and Affiliations
Robert Kuthan, Łukasz Chabros, Anna Sawicka-Grzelak, Grażyna Młynarczyk
Keywords
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