Identification of the grass family (Poaceae) by using the plant dna barcodes rbcl and matK
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2016, Vol 8, Issue 5
Abstract
Due to phenotypic plasticity, with few distinguishable and many overlapping characters, grasses are very difficult to identify by morphological characters, The current research evaluated how standard plant barcode rbcl and matK can help in floristic evaluation of. grasses belonging to a not well studied flora in district Dera Ghazi Khan, Punjab, Pakistan. In this study, 54 specimen belonging to 24 species of Poaceae were examined. Species wise sequencing success for rbcl and matK was 100% and 66.67% respectively. The determination of intra and inter-specific divergence and phylogentic analysis by reconstruction of neighbor joining trees were carried out. The results demonstrated that individually both rbcl and matK totally failed in discrimination of congeneric species. In neighbor joining phylogenetic analysis both rbcl and matK provided well resolved monophyletic tree with weak bootstrap threshold value. While in combination (rbcl+matK) both marker provided well resolved monophyletic tree with strong bootstrap threshold value.
Authors and Affiliations
Saadullah, Zaheer-ud-din Khan, Muhammad Ashfaq, Zaib-u-Nisa
Keywords
Related Articles
- EP ID EP38816
- DOI -
- Views 158
- Downloads 0
Effects of storage methods, storage duration and different geographical locations on quality of stored wheat (Triticum aestivum) in sindh, Pakistan
Wheat grain stored in traditional structures was sampled from four selected districts of Sindh from July 2013 to July 2014 at 3 months interval and was analysed for quality deterioration over time. The maximum ambient...
Investigation the role of industry in polluting the groundwater aquifers (Case study: Ardabil aquifer, Northwest of Iran)
A large part of water consumption in the world, especially in drinking part, provided from the groundwater resources. In many cases, groundwater pollution, is diagnosed when the decontamination of the aquifer is almost...
Functional response of Trichogramma brassicae Bezdenko to Anagasta kuehniella (Zeller) and Phthorimaea operculella (Zeller) eggs
The parasitoid Trichogramma brassicae Bezdenko is used for inundative releases in biological control programs against lepidopteran pests in agro-ecosystems. Study on the functional response of natural enemies is one of t...
Impact of waste water of a pulp and paper industry on surrounding environment
To fulfill the demands of ever increasing population it becomes essential to escalate the industrial development in our country.Pulp and paper industry is considered to be one of the most polluting industries. This indus...
Determining frequency of ionic, type, faciesand water contaminants of Karoon river
The changes in river ecosystem and fading away the species of organisms lead to risk of dearth of high quality water resources and to harmful impacts resulting from using contaminant waters on human beings. In order tha...