Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of Artemisia campestris from Algeria
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 2
Abstract
Objectives: Our study aimed to investigate the chemical profile of hydroalcoholic extract of Algerian Artemisia campestris and its antioxidant activity. Materials and Methods: The hydroalcoholic extract of Algerian A. campestris was investigated for its phenolic constituents using high performance liquid chromatography (HPLC)-diode array detection (DAD)-electrospray ionization (ESI)-mass spectrometer (MS)/MS. The in vitro antioxidant activity and total phenolic content were also evaluated via oxygen radical absorbance capacity and Folin–Ciocalteu assays, respectively. Results: HPLC-DAD-ESI-MS/MS analysis revealed that the main tentatively identified compounds were caffeoylquinic acid isomers, flavonoids, and benzoic acid derivatives. Additionally, the hydroalcoholic extract exhibited a promising antioxidant activity value of 120.5±10.4 μmol Trolox equivalent antioxidant capacity/g dry weight (DW), and a strong correlation exists between this activity and the total phenolic content value of 102.09±1.65 mg/g gallic acid equivalents DW. Conclusion: The hydroalcoholic extract of A. campestris is a promising candidate for the production of naturally occurring antioxidant agents.
Authors and Affiliations
Boulanouar BAKCHICHE, Abdelaziz GHERIB, Maria Rosário BRONZE, Mosad A. GHAREEB
Keywords
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