IL-8 mRNA and Serum Levels in Vitiligo Patients: A Case Control Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 11
Abstract
Introduction: Many theories are suggested to explain aetiology of vitiligo, such as autoimmune, genetic and biochemical. Interleukin-8 (IL-8) is a pro-inflammatory chemokine which is evaluated in the pathogenesis of some skin diseases, like psoriasis, however, in vitiligo, few studies were reported regarding this issue. Aim: The aim of the present study was to investigate serum level of IL-8 and IL-8 mRNA in patients with different types of vitiligo to validate its role in aetiopathogenesis of vitiligo. Materials and Methods: This case-control study was conducted on 39 vitiligo patients and 15 age and gender matched healthy controls. The participants were selected from Dermatology Outpatient Clinic, Menoufia University Hospitals from October 2014 to October 2015. They were subjected to clinical history, examination and full general examination. Detailed dermatological examination including VASI score was applied. IL-8 serum level was measured by ELISA and IL-8 mRNA was quantitated by Real Time-PCR. The statistical analysis was done using SPSS, version 20.0. Mann Whitney U test was used to compare two quantitative not normally distributed. Chi-square test (χ2 ) was used to study association between two qualitative variables. Spearman correlation test was applied to assess correlation between two continuous quantitative variables. A p≤0.05 was considered significant. Results: IL-8 serum level and mRNA concentration mean values were significantly elevated in vitiligo patients (26.25±43.28 pg/ mL and 8.48±11.92 ng/mL) than controls (0.57±0.50 pg/mL and 0.60±0.32 ng/mL) (p=0.002, for both), and they showed significant positive correlation with each other (r=0.622; p≤0.001). Moreover, both of them revealed significant high values in localised vitiligo (62.20±74.39 pg/mL and 12.42±13.85 ng/mL) than generalised (18.39±29.49 pg/mL and 7.61±11.52 ng/mL) (p≤0.000 and p=0.004), respectively. Conclusion: Serum IL-8 chemokine and its mRNA increased significantly in vitiligo patients indicating that it may have a dynamic role in vitiligo development and participate in its pathogenesis.
Authors and Affiliations
Azza Gaber Farag, Alaa Hassan Marae, Mona Salah Eldin Habib, Reda Zakaria Mahfouz, Nada Farag Elnaidany, Salwa Ibrahim Youssef, Sherifa Morsy El-Feky
Keywords
Related Articles
- EP ID EP366990
- DOI 10.7860/JCDR/2017/30392.10857
- Views 64
- Downloads 0
Successful Management of the Masochistic Habit in a Child with Santovuori-HaltiaHagberg Disease (Infantile Neuronal Ceriod Lipofuscinoses)
Self-injurious behaviours are usually related to paediatric patients with mental retardation. The management of such patients is quiet challenging to the paediatric dentists because of the difficulty to communicate with...
Adenomatoid Tumours: A Short Series with Literature Review
Adenomatoid tumours are hormone independent tumours of mesothelial origin. These are distinctive benign neoplasms usually localised in the epididymis in males and myometrium in females. They are uncommon and account for...
A Rare Case of Twinning Involving Primary Maxillary Lateral Incisor with Review of Literature
Twinning is referred to the development of two separate teeth that arose from the complete separation of one tooth bud. To the best of our knowledge very few cases of twinning in primary or permanent dentition have been...
Use of Impella 2.5 in Patient with Multi-Vessel Percutaneous Coronary Intervention and Cardiogenic Shock
Images in Medicine
Anthropometric Basis of Vertical Jump Performance: A Study in Young Indian National Players
Introduction: Vertical Jump (VJ) is a good measure of athletic performance and occupational activities. Earlier studies reported conflicting results on anthropometric influence. Aim: To evaluate the relationship between...