Immobilization and Biochemical Properties of Purified Xylanase from Bacillus amyloliquefaciens SK-3 and Its Application in Kraft Pulp Biobleaching
Journal Title: Journal of Clinical Microbiology and Biochemical Technology - Year 2016, Vol 2, Issue 1
Abstract
In the present study, we studied the production and immobilization xylanase from Bacillus amyloliquefaciens strain SK-3. Isolate produced highest xylanase activity (56.2±1.6 IU/mol) at pH=8 and 40°C after 48 h incubation in presence of 1% wheat bran. Immobilization studies of purified xylanase showed that 3.0% sodium alginate and 0.2 M calcium chloride was found to be optimum. Characterization of immobilized beads by SEM and FTIR showed significant changes on the surface morphology and structure. The immobilization increases the time reaction for xylan degradation from 15-30 min and pH activity 8.0 to 9.0 whereas temperature 60-70°C with reference to free enzyme. After immobilization, thermostability of enzyme increased and retained more than 70% of its original activity after 5 h at 50°C as compared with free enzyme which showed only 20% of residual activity. Also, immobilized enzyme showed better storage stability and reusability. Overall performance of immobilized enzyme has attractive biochemical properties that make it a potential promising candidate for application in the kraft pulp pretreatment.
Authors and Affiliations
Kumar Sharad, Haq Izharul, Yadav Ashutosh, Prakash Jyoti, Raj Abhay
Keywords
Related Articles
- EP ID EP353351
- DOI 10.17352/2581-527X.000012
- Views 90
- Downloads 0
Immobilization and Biochemical Properties of Purified Xylanase from Bacillus amyloliquefaciens SK-3 and Its Application in Kraft Pulp Biobleaching
In the present study, we studied the production and immobilization xylanase from Bacillus amyloliquefaciens strain SK-3. Isolate produced highest xylanase activity (56.2±1.6 IU/mol) at pH=8 and 40°C after 48 h incubation...
Hepatopulmonary Syndrome and Portopulmonary Hypertension in the Same Patient
Hepatopulmonary syndrome (HPS) and portopulmonary hypertension (POPH) are the most common pulmonary vascular complications in patients with liver disease. We present a 71-year old woman with alcoholic cirrhosis and 6 mon...
Biochemical parameters of Common Carp (Cyprinus Carpio) exposed to Cadmium change to the leaf extract of Abutilon indicum
Heavy metals pollution in aquatic ecosystem is global issue due to persistence and continuous accumulation of these pollutants in aquatic environment. This results in excess release of heavy metals such as cadmium, coppe...
Halotolerant Co-Cultured Bacterial Strains used in Degradation of Tannin Isolated from Tannery Industry Contaminated Sites
The residual tannins discharged from tanning units of the leather industry have an adverse effect on living organism and causes serious environmental pollution. Biological degradation is an important mechanism of organic...
Listeria Monocytogenes as a Foodborne Pathogen: Biocontrol in Foods using Lytic Bacteriophages
Foods are playing a significant role in human infections because they are frequent vehicles of some human pathogens, which can spread in a short time to all the animals and are associated with cross contamination during...