Immobilization and Thermostability of Manganese Peroxidase from Trichoderma harzianum
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 2
Abstract
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best carbon and nitrogen sources were sucrose and yeast extract. The best production of MnP was formed at the 5th day of growth using 1 and 5 mM of Mn2+. The enzyme was immobilized on luffa, ceramic, alginate and chitosan and the percentages of immobilization were 40.1%, 15.9%, 82.1% and 91.3% respectively. The immobilized enzyme was expressed better stability at 50°C specially that immobilized at chitosan. Thus, the present results suggest that Trichoderma harzianum is a good candidate for production of manganese peroxidase which can be used in many biotechnological applications.
Authors and Affiliations
Hamed M. El-Shora, Mohsen E. Ibrahim, Mohamed R. Elmekabaty
Keywords
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- EP ID EP316031
- DOI 10.9734/MRJI/2017/35754
- Views 79
- Downloads 0
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