Immobilization and Thermostability of Manganese Peroxidase from Trichoderma harzianum
Journal Title: Microbiology Research Journal International - Year 2017, Vol 21, Issue 2
Abstract
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best carbon and nitrogen sources were sucrose and yeast extract. The best production of MnP was formed at the 5th day of growth using 1 and 5 mM of Mn2+. The enzyme was immobilized on luffa, ceramic, alginate and chitosan and the percentages of immobilization were 40.1%, 15.9%, 82.1% and 91.3% respectively. The immobilized enzyme was expressed better stability at 50°C specially that immobilized at chitosan. Thus, the present results suggest that Trichoderma harzianum is a good candidate for production of manganese peroxidase which can be used in many biotechnological applications.
Authors and Affiliations
Hamed M. El-Shora, Mohsen E. Ibrahim, Mohamed R. Elmekabaty
Keywords
Related Articles
- EP ID EP316031
- DOI 10.9734/MRJI/2017/35754
- Views 87
- Downloads 0
Assessing the Impact of Antiretroviral Therapy on CD4, Hemoglobin Level and Weight in HIV Infected Children (0-15 Years) Residing in Nkambe, North West Region, Cameroon
Background: Antiretroviral therapy (ART) has greatly decreased the mortality and morbidity of children living with HIV by reducing the viral load, increase CD4 count thus improving the health of HIV clients. This study s...
Microbial Count of Non-sterile Pharmaceutical Products Sold in Pakistan
Aims: To explore the microbiological quality of tablets in blister-packed, manufactured and marketed in Pakistan in order to assess Good Manufacturing Practice (GMP) by national and multinational pharmaceutical industrie...
In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda
Aims: To determine antibacterial activity of Ocimum suave essential oils against bacterial uropathogens. Study Design: A cross sectional and experimental study. Place and Duration of Study: Six selected hospitals in Bush...
In vitro Antibacterial Activity of the Extracts of Peperomia pellucida (L)
Background: Peperomia pellucida is an economic plant grown in West Africa. Aim: We investigated the phytochemical and antimicrobial activity of N-hexane, Ethyl acetate, and Ethanol extract of Peperomia pellucida whole p...
Evaluation of Antibiotic Biosynthetic Potential of Actinomycete Isolates to Produce Antimicrobial Agents
Aim: To assess the antibiotic biosynthetic potential of actinomycete isolates from rhizospheric soil samples collected from Ngaka Modiri Molema district in North West Province of South Africa. Study Design: The analysis...