Immobilization of Human Sperms by Gossypol Incorporated into l-Ascorbyl Palmitate Coagel
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 14, Issue 5
Abstract
Gossypol and its derivatives have interestingly showed potential contraceptive effects by acting as spermicidal agents concomitantly having other versatile biological activities against numerous human pathogenic infections, to get rid of its systemic toxicity, gossypol was incorporated into l-Ascorbyl Palmitate (l-AP) coagel as topical formulation. The in vitro immobilizing effect of gossypol incorporated into l-Ascorbyl Palmitate coagel on hu-man sperms was investigated. The spermatozoal motility and straight line velocity (VSL) analysis was conducted by using Computer Assisted/Aided Sperms Analyzer-motility (CA-SA-Mot.) system on 30 human semen samples after exposure to 0.1%, 0.2%, 0.3%, 0.4% and 0.5% (w/w) gossypol-containing coagels. There appeared a lag time up to around 5minutes without any detectable effect followed by a non-immediate and quick immobi-lization effect through which a great number of progressively motile spermatozoa were completely seen immobilized after an average minimum period of 10minutes. Two out of thirty subjects, few sperms with weak tail shake-like motions were seen but without forward progression even after 30 minutes of exposure. The deep decrease in percent-age of motile sperms was dependent to gossypol concentration and duration of exposure, spermatozoal progressive straight line velocity decreased rapidly and remained low until the motility ceased. The 0.2% gossypol coagel showed the best immobilizing effects at reasonable activity time promising a better spermicidal activity required for multipurpose contraceptive formulations for future use for control of both pregnancy and some sexually transmitted infections.
Authors and Affiliations
Mutabazi Francois, Tang Luhong, WANG Honghua
Keywords
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- EP ID EP585998
- DOI 10.26717/BJSTR.2019.14.002621
- Views 153
- Downloads 0
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