Immobilization of Laccase in poly (Vinyl Alcohol)-Calcium Alginate Beads
Journal Title: Cumhuriyet Science journal - Year 2018, Vol 39, Issue 3
Abstract
Laccase enzyme (L) obtained from Tramates versicolor was entrapped into polyvinyl alcohol–calcium alginate (PVA-CaAlj) beads. Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) values were found to be 1.70x10-2 mM and 2.08x10-3 mM.min-1 for free enzyme respectively. Km and Vmax values were found as 2.87x10-2 mM and 5.30 x 10-3 mM.min-1 for entrapped enzymes respectively. Optimum pH was determined as 5.0 and 6.0 and optimum temperature determined as 40oC and 45oC for free laccase and entrapped laccase respectively. After 30 days of storage at 4 oC free laccase retained 60 % of its original activity. Also after 30 days of storage at 4 oC, entrapped enzymes were retained 85 % its original activity. Immobilized enzyme was used repeatedly 10 times, were retained 75% of its original activities.
Authors and Affiliations
Haydar ALTINOK
Keywords
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