Immuno-Histochemical Study of P16INK4A in Cervical Intraepithelial Neoplasia and Cervical Cancer
Journal Title: Journal of Medical Science And clinical Research - Year 2017, Vol 5, Issue 1
Abstract
Background: Cervical cancer is caused by human papilloma virus (HPV) strains 16 and 18. One of the consequences of viral genomic integration into the host cell is up regulation of p16INK4A, a cyclin dependent kinase inhibitor. This implies that detection of p16INK4A may be used as a surrogate marker of integration of high risk HPV into cervical epithelium and transition to malignant transformation. Aim: The aim of this study is to assess utility of p16INK4A immunopositivity and evaluate the usefulness of this biomarker in the diagnosis of CIN and cervical cancer. Materials and Methods: 65 cervical biopsy/ hysterectomy specimens of LSIL, HSIL and SCC were studied for H&E and compared with IHC staining. Subsequently, the immunohistochemically stained slides were graded on a 0-3 grading scale. Results: 65 evaluated cases on histopathological examination showed that 23 (35.4%) cases were LSIL, 8 (12.3%) cases were HSIL and 34 (52.3%) cases were SCC of uterine cervix 23 cases of LSIL were studied for p16INK4A over expression in which 20 (87%) cases showed grade 1 staining and 3 (13%) cases showed grade 2 staining 8 cases of HSIL were studied for p16INK4A over expression in which 1 (12.5%) case showed grade 2 staining and 7 (87.5%) cases showed grade 3 staining 34 cases of SCC were studied for p16INK4A over expression in which 3 (8.8%) cases showed grade 2 staining and 31 (91.2%) cases showed grade 3 staining Conclusion: The present study concludes that p16INK4A is a specific marker for dysplastic and neoplastic alteration in the cervical epithelium and thereby significantly improves cervical pre-cancer and cancer detection.
Authors and Affiliations
Dr Sharath Kumar H. K
Keywords
Related Articles
- EP ID EP246266
- DOI -
- Views 81
- Downloads 0
Giant Gastric Gastrointestinal Stromal Tumor Encasing Splenic Hilum- A Case Report
Gastrointestinal stromal tumour (GIST) is a rare tumour of GI tract (0.2%). GIST arise from Interstitial cell of Cajal which intercalates between smooth muscle cells and intramural neuron. Stomach is the most common site...
Assessment of Janani Suraksha Yojana (JSY) Component Under NRHM in Selected Districts of Kashmir Valley: A Descriptive Study
National Rural Health Mission launched a safe motherhood intervention named Janani Suraksha Yojna in 2005 to reduce maternal and infant mortality by promoting institutional deliveries. The objective of the study was to a...
Assessment of Vein Intima-Media Thickness in Determining Maturity of AV Fistula
Vascular access is a hemo-dialysis patient’s lifeline. Two types of permanent vascular access are designed which include the arterio-venous fistula and the arterio-venous graft. After surgical creation, arteriovenous fis...
To Study and Evaluate the Incidence o f Cutaneous Adverse Drug Reaction (CADRS ) in t he Patients At tending Dermatology Department o f A Tertiary Care Teaching Hospital
Adverse cutaneous drug reactions (CADRs) are commonly reported type of ADRs and are caused by a wide varieties of drugs. The clinical patterns of adverse cutaneous drug reactions and the drug responsible for them is ch...
AMITRAZ Poisoning: Uncommon and Overlooked Human Toxicity of a Common Pesticide
Amitraz is a widely used pesticide worldwide. Chemically it is a triazapentadiene, having predominant effect as centrally acting α2 adrenergic agonist. Human toxicity of amitraz is an unusual occurrence mostly due to acc...