Immunobiological indicators in horses with latent rhinopneumonia
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2019, Vol 34, Issue
Abstract
Introduction. The causative agent of rhinopneumonia is herpesvirus of the first type (HSV-1) that one of the main causes of respiratory diseases and abortions in horses. The disease causes significant economic losses to the horse breeding, which leads to loss of reproductive capacity of mares, culling of valuable breeding animals and costs for veterinary and sanitary measures. In accessible reference sources we did not find any reports regarding the study of the immunological status of horses with the latent course of rhinopneumonia. The goal of the work was to investigate the complex of immunobiological parameters of horses in the dynamics of the latent course of rhinopneumonia. Materials and methods. Neutralization reaction for the specific antibodies determination in horses sera was carried out on a subculture of chick embryo cells (CEC) using 96-well polystyrene microplates with virus dose of 100 TCID 50/0.3 cm3 titrated on cell culture. Hematologic studies (determination of the number of erythrocytes, hemoglobin, leukocytes) and the content of total protein, albumin, and fractions of globulins in horses’ sera were carried out according to generally accepted methods. Results of research and discussion. It was found that with the development of the infectious process, a decrease in the content of total protein, albumins, α-globulins, albumin-globulin ratio, erythrocytes, hemoglobin, as well as an increase in the number of leukocytes, the content of γ- and β-globulins was observed. We assume that one of the reasons that caused the change in immunological, biochemical and hematological parameters in horses is the immunosuppressive effect of the viral pathogen. It is not excluded the development of pathological changes in sick animals organism. Conclusions and prospects for further research. The latent rhinopneumonia of horses is accompanied by a decrease in hematological and immunobiochemic parameters caused by the immunosuppressive effect of the viral agent, and a high titer of neutralizing antibodies from 1:80 to 1: 160 and higher along with a simultaneous decrease in the albumins and immunoglobulins ratio (0.90±0.10 RU) is a prognostic criterion for the identification of horses with an increased risk of disease development. In the future, to study the development of the infectious process, it is necessary to study the reaction of immune cells.
Authors and Affiliations
P. Kryvoshyia, O. Rud
Keywords
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- EP ID EP555172
- DOI 10.31073/vet_biotech34-10
- Views 95
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