Immunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography
Journal Title: Iranian Journal of Blood and Cancer - Year 2014, Vol 6, Issue 2
Abstract
Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichment of IgA, ion exchange chromatography using Sephadex DEAE A-50 on a Pharmacia glass column was carried out. Results: By fractionation of pooled human plasma and then treatment with caprylic acid, the amount of immunoglobulins showed an increase from 17% to 80% measured by immunoturbidimetric method. Then the ion exchange column chromatography by Sephadex DEAE A-50 was carried out. In washing solution 65 % of loaded IgG and only 17% of loaded IgA were found. In elution solution 32% of loaded IgG and 79% of loaded IgA were obtained, which showed the ion exchange chromatography is a promising method for preparation of enriched IgA solution. Conclusion: Because of the similarity between IgA and IgG, techniques based on net charge and molecular differences would not give a high yield in IgA isolation. As IgA has a negative charge at the pH 8.1, Sephadex DEAE A-50 which is able to bind proteins at relatively high ionic strength was chosen for ion exchange chromatography. After column ion exchange chromatography, the concentration of obtained IgA was three times more enriched in comparison with the concentration of IgA in normal plasma. Keywords: IgA, IgG, plasma, chromatography.
Authors and Affiliations
Mir Kamran Mousavi Hosseini, Mojgan Pourmokhtar, Mohammad Ali Jalili, Saleh Nasiri
Keywords
Related Articles
- EP ID EP364748
- DOI -
- Views 64
- Downloads 0
Infusible Platelet Membrane versus Conventional Platelet Concentrate: Benefits and Disadvantages
Blood transfusion centers are under considerable pressure to produce platelet concentrates with a shelf life limit of 3 to 5 days. Many approaches have been investigated experimentally to produce new hemostatically activ...
Multiple Myeloma Presenting as Respiratory Stridor
Extramedullary plasmacytoma occurs in 18% of patients with multiple myeloma. Laryngeal involvement in multiple myeloma is rare, and only a few cases have been reported. We present a case of a 44-year-old women with multi...
Case Series of Hepatoblastoma
Background: In the past 20 years, a dramatic improvement in the prognosis of patients with hepatoblastoma (HB) has been achieved by combining surgery and chemotherapy in several national and international trials. Materia...
Clinical application of plasma derived medicines: current situation and future trend
This article has no abstract.
Antineoplastic Effect of Fenugreek (Trigonella Foenum Graecum) Seed Extract against Acute Myeloblastic Leukemia Cell Line (KG-1)
Background: Treatment of cancer patients using conventional chemotherapy causes serious side effects and, at best, merely extends the patient’s lifespan by a few years. The potential of alternative therapies may therefor...