Immunohistochemical Detection of Helicobacter pylori in Oral Squamous Cell Carcinoma
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 6
Abstract
Abstract: Head and neck carcinomas are biological heterogeneous group of cancers, of which oral cancer is the most common. Ninety percent of oral cancers are squamous cell carcinomas originating from the mucosal epithelium. Oral carcinogenesis is a multifactorial process where numerous risk factors are involved. A connection between bacterial infection and carcinogenesis is convincing and is of increasing interest. Helicobacter pylori (H.pylori) are flagellated, gram negative, spiral, microaerophilic bacteria and deemed by the World Health Organization and the International Agency for Research on Cancer as a class I human carcinogen. In addition to its role in causing gastritis, gastric ulcer, duodenal ulcer and adenocarcinoma of stomach, H.pylori has been found in the oral cavity in patients with halitosis, apthous stomatitis and periodontal diseases. However, there are limited studies available in literature of H.pylori in oral squamous cell carcinoma (OSCC) and its possible role in oral carcinogenesis. Therefore the aim of this study is to detect and establish a relationship between H.pylori and OSCC. Thirty paraffin embedded tissue blocks of clinically diagnosed and histopathologically confirmed cases of OSCC and ten of normal buccal mucosa were sectioned and stained with Hematoxylin and Eosin. The one subsequent serial section is immunohistochemically stained with anti human helicobacter pylori antibody. The presence of H.pylori is assessed and any association between presence of H.pylori and OSCC was analyzed. The study data was analysed using SPSS (Statistical Package for Social Sciences) software V.22, IBM, Corp. Statistical Analysis was done using Chi Square test and Mann Whitney U test. The level of significance [P-Value] was set at P<0.01. Chi Square test was used to find the association between the prevalence of H.Pylori between OSCC & Control group and it was found to be stastically significant (at P<0.001). Chi Square test showed significant differences between H. pylori positivity and different tissue types i.e in epithelium, lamina propria, inside the blood vessels and salivary gland duct and in the muscle layer of OSCC. There is a positive relation between the presence of H. pylori and OSCC. Therefore, early detection and eradication of H. pylori in high-risk patients are suggested.
Authors and Affiliations
Dr. Kavita Gupta, Dr. Leeky Mohanty, Dr. Chaitanya N Babu, Dr. Sweta Dash
Keywords
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