Immunohistochemical Expression of Caspase-3 in Psoriasis
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 7
Abstract
Introduction: Psoriasis is a persistent chronic immune-mediated, relapsing, inflammatory and hyper proliferative skin disorder with genetic predisposition. Psoriasis can be considered as a T-cell mediated disease, with a complex role for a variety of cytokine interaction between keratinocytes and T-lymphocytes. Caspase-3 is an enzyme that plays a key role in apoptosis; it is a member of the family of cysteinyle aspartate specific proteases. Aim: To evaluate the expression of caspase-3 in Egyptian psoriasis patients and its role in apoptosis of keratinocytes. Also, to correlate this expression with the clinicopathological parameters in order to identify the possible hypothesized role of caspase-3 in the pathogenesis of psoriasis. Materials and Methods: This was a case-control study conducted on patients suffering from chronic plaque psoriasis. A total of 20 psoriasis patients and 10 controls were selected from outpatient clinic of Dermatology, Menoufia University Hospital, between the period of October 2014 and January 2016. From each patient and control, a punch biopsy was taken. Evaluation of H&E stained sections and caspase-3 expression was done using standard immunohistochemical techniques. Non-parametric chi-square test, MannWhitney U test, Kruskal Wallis test and Spearman’s coefficient test were the statistical tests used. Results: High caspase-3 H score was significantly in favour of psoriatic group in comparison with the control group. On the contrary, in the dermis, caspase-3 was significantly higher in skin adnexa while completely absent in the psoriatic group. Strong caspase-3 expression was significantly in favour of high PASI score, early onset lesions and lesions in the extremities. Significant positive correlation was found between caspase-3 percent and PASI score (r= +0.53, p-value=0.03). Conclusion: Caspase-3 over expression in the psoriatic lesion proposes a potential role in the pathogenesis of psoriasis. Positive correlation between the caspase-3 expression and the early onset psoriatic lesion located in the extremities implies a possible poor prognostic impact of caspase-3 over expression.
Authors and Affiliations
Shaimaa M. M. Bebars, Dalia Rifaat Al- Sharaky, Mohammed A. Gaber, dina ragab afify
Keywords
Related Articles
- EP ID EP353437
- DOI 10.7860/JCDR/2017/25609.10145
- Views 64
- Downloads 0
Non-milk based Local Therapeutic Feed Plus Home-based Diet as Compared to Home-based Diet Alone for Nutritional Rehabilitation of Severe Acute Malnutrition Following Discharge: A Randomised Clinical Trial
ABSTRACT Introduction: Recovery of Patients of Severe Acute Malnutrition (SAM) after discharge from Nutrition Rehabilitation Centre (NRC) is unsatisfactory on Home-based diet. Authors, therefore, compared recovery of chi...
Incidence and Risk Factors for Prolonged Stay in Children Hospitalised with Pneumonia
ABSTRACT Introduction: Lower Respiratory Tract Infection (LRTI) is one of the common causes of morbidity and mortality in children and is responsible for hospitalisation. Aim: The current descriptive epidemiological stud...
A Rare Case of Poorly-Differentiated Sertoli Leydig Cell Tumour of Ovary with Mesenchymal Heterology
Abstract Sertoli–Leydig Cell Tumours (SLCT) accounts for less than 0.5% of all ovarian neoplasms. Presence of mesenchymal heterologous elements in a poorly differentiated SLCT is extremely uncommon. It not only causes di...
A Case Report of Rare Carcinoma Ex Pleomorphic Adenoma of Submandibular Gland and its Detailed Description
A 65-year-old female patient reported with a chief complaint of swelling in the left lower region of the jaw since two years. On clinical examination, the swelling was present on the mandibular left posterior region and...
Role of EEG in Diagnosing Abdominal Epilepsy Patients
ABSTRACT Introduction: Abdominal Epilepsy (AE) is an uncommon cause of paroxysmal abdominal pain. It is very challenging to diagnose a case of AE. This entity is less recognised as there is paucity in report and its lite...