IMMUNOHISTOCHEMICAL STUDY OF E-CADHERIN AND P53 PROTEIN EXPRESSION IN FEMALE MAMMARY CARCINOMAS USING A TISSUE MICRO ARRAY TECHNIQUE GEZIRA-CENTRAL SUDAN
Journal Title: World Journal of Pharmaceutical and Medical Research - Year 2017, Vol 3, Issue 3
Abstract
Objective: To improve the breast cancer diagnostic and prognostic capabilities by application of immunohistochemical technique for assessment of expression of p53 and E-cadherin, To assess the expression of E-cadherin and p53 protein markers in breast invasive ductal and lobular carcinomas in FFPE (formalin fixed paraffin embedded) tissue. Material and Methods: The material of this study consisted of 41 FFPE blocks of primary breast carcinoma specimens. Multiple sections from all paraffin blocks were cut at 4 microns thickness and stained: 1.Sections from tumors and surrounding breast tissue were stained by hematoxylin and eosin stain for histopathological evaluation. 2.Immunohistochemical staining by E-cadherin monoclonal antibody. 3.Immunohistochemical staining by p53 monoclonal antibody. Results: Out of the 41 cases examined 26cases (63.4%), were positive, while 13 cases (36.6%), were negative, studied 5 cases (12.2%), were negative while 36 cases (87.8%) were positive. Conclusion: Immunohistochemical detection of p53 and E-cad has been demonstrated to be a reliable and easily performed on paraffin embedded tumor specimens, p53 immunostaining is a reliable method for detection of aggressiveness of ductal breast carcinomas and could be considered to have a role in metastatic process in breast carcinoma, p53 protein has a role in E-cad expression in breast carcinomas.
Authors and Affiliations
Dr. Abbas A. A.
Keywords
Related Articles
- EP ID EP651923
- DOI -
- Views 213
- Downloads 0
BEHAVIORAL PROFILE OF INTELLECTUALLY DISABLED PATIENTS ATTENDING A TERTIARY CARE CENTER IN INDIA
Background: Intellectual disability (ID) is a common problem complicated by behavioral and physical comorbidity. The common behavioral problems are related to conduct, ADHD, aggression or agitation and emotion. There dat...
KINETICS OF DECOMPOSITION AND OPTICAL OF RUBIDIUM SOAPS
Isothermal decomposition of solid Rubidium soaps, (caprylate and caprate) as a function of temperature was studied. Thermal decomposition can be expressed in quite agreement with Prout-Tompkins equation and used to estim...
ACUTE DISSEMINATED ENCEPHALOMYELITIS OR SUBACUTE SCLEROSING PANENCEPHALITIS? A CASE REPORT
Subacute Sclerosing Panencephalitis (SSPE) is a delayed and fatal complication of measles. It is a slowly progressive encephalitis that usually occurs 6 to 10 years after measles infection, and progresses over 12 to 18 m...
AUDIT OF CHILDHOOD TETANUS PATIENTS ADMITTED IN TERTIARY CARE HOSPITAL
Background: Tetanus is an important cause of morbidity and mortality in developing countries though it is rare in developed countries. Childhood tetanus has high mortality and is preventable. Knowledge about the need of...
BIOLOGICAL CONTROL OF AQUATIC MACROPHYTES AND ECOLOGICAL IMPLICATION: A REVIEW
The study reviews the biological control of aquatic macrophytes and its ecological implications to provide information on various future challenges in water quality management and aquaculture. Aquatic macrophytes cause v...