Immunohistochemistry: A Review of Practical Procedure
Journal Title: Nepal Journal of Neuroscience - Year 2009, Vol 6, Issue 2
Abstract
Immunohistochemistry (IHC) is a simple, yet a highly essential, research and diagnostic tool in the field of molecular biology and medical science. It is a procedure of antigen detection in a tissue by using the principle of antigen antibody reaction. This is the technique by which different tumor markers are detected in tumor samples. The method of staining tissue sections and detecting antigen in the tissue is called immunohistochemistry. Similar process to detect antigen in a cell is called immunocytochemistry. Grossly there are 3 steps in IHC namely slide preparation, slide staining and slide interpretation. More precisely the whole procedure can be divided into different sections as follows- deparaffinization and rehydration, activation of antigen, endogenous peroxidase blocking, primary antibody incubation, secondary antibody incubation, antibody detection, counterstaining, and slide mounting. It takes about 6 hours to complete the immunostaining and can be divided into two parts and done in two consecutive days. The purpose of this article is to review the basic principle and simplify the procedure of IHC. The target group is the young medical practitioners who have every scope of carrying out different research works.
Authors and Affiliations
Prabin Shrestha, MD, PhD
Keywords
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