Immunomodulation Potential of Andrographis Paniculata and Tinospora Cordifolia Methanolic Extracts in Combination Forms

Journal Title: International Journal of Pharmacological Research - Year 2016, Vol 6, Issue 1

Abstract

Objective: This work mainly focuses on the immunomodulatory effect of different drug combinations (DC-I to DC-V) of methanolic extracts of aerial parts of Andrographis paniculata (AP) and mature stems of Tinospora cordifolia (TC). Materials and Methods: The coarse powder (40-mesh) of shade dried aerial parts of AP and mature stems of TC (500g each) was subjected separately tosuccessive extraction with 1000ml each of petroleum ether (60-80oC) followed by methanol. The doses of DC-I to DC-V were selected and were administered orally at doses of 200 mg/kg body weight to Albino Wistar rats and compared with the control group and standard drug i.e. cyclophosphamide (100mg/kg) respectively. Results: Results suggest that although all the five tested combinations (DC-I to DC-V) prepared using different proportion of methanol extracts of A. paniculata and T. cordifolia (100:Zero, 75:25, 50:50, 25:75 and Zero:100) showed a significant increase in macrophase count, neutrophil adhesion(NA), haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) response. In rats immunized with sheep RBC, DC-I to DC-V enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the footpad thickness response to sheep RBC in sensitized rats on chosen experimental models. Conclusion: The values of macrophase, NA, HA, DTH and cellular responses of combination DC-II at a 200 mg/kg body weight were statistically significant as compared to other combinations. Combination DC-II exhibited best immunostimulant potential, indicating that it has promising immunomodulatory activity, as proposed from commercial point of view.

Authors and Affiliations

Rajesh Yadav, N Yadav, M D Kharya

Keywords

Related Articles

Antioxidant Activity of Ethanolic Extracts of Callicarpa Linata Leaf

Antioxidants are our first line of defense against free radical damage, and are critical for maintaining optimum health and wellbeing. Catalase (CAT), superoxide dismutase (SOD), and thiobarbituric acid reactive Substanc...

Effect of Gymnema sylvestre on the pharmacokinetics and pharmacodynamics of 0.5mg & 0.6mg Glibenclamide in diabetic rats

Traditional medicines derived from medicinal plants are used by about 60 per cent world population. Diabetes is an important human ailment officiating many from various walk of life in different countries including India...

Agranulocytosis induced by anti-tubercular drugs, Isoniazid (INH) and Rifampicin (R) A rare case report

Background: Tuberculosis is a chronic granulomatous infection that has caused a high morbidity and mortality over decades. Tuberculosis treatment has many side effects due to long duration and compliance problems that mi...

An ethnobotanical study of medicinal plants used by the Masaai people of Losho, Kenya

Objective: An ethnobotanical survey on the medicinal plant species in Losho, Narok County, Kenya was conducted in order to document traditional medicinal knowledge and application of medicinal plants. Materials and Meth...

Histological Changes in Epididymis of Albino Rats by Graded Doses of Cyclophosphamide

Background: Cyclophosphamide is widely used in the treatment of various neoplastic diseases and diseases associated with altered immunity. Higher doses used for longer duration effects many organs like urinary bladder, l...

Download PDF file
  • EP ID EP435217
  • DOI 10.7439/ijpr.v6i1.2822
  • Views 61
  • Downloads 0

How To Cite

Rajesh Yadav, N Yadav, M D Kharya (2016). Immunomodulation Potential of Andrographis Paniculata and Tinospora Cordifolia Methanolic Extracts in Combination Forms. International Journal of Pharmacological Research, 6(1), 29-40. https://europub.co.uk/articles/-A-435217