Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells

Journal Title: Turkish Journal of Immunology - Year 2017, Vol 5, Issue 1

Abstract

Objective: The purpose of this study was to explore the effect of propolis extract on CD8+ and CD4+CD25+ regulatory T cell populations and granzyme expression in both cell populations, as propolis has been suggested as an immunomodulatory agent. Materials and Methods: PBMC containing 1x106 cells/ml from cervical cancer stage 3 patients were isolated and cultured with and without propolis extract. The cells were plated in a 24-well plate with RPMI medium supplemented with 10% FBS, 10 U/ml penicillin, 100 U/ml streptomycin, 2 mM L-glutamine and 50 μM 2-ME. Propolis extract was added (5, 10, 25 and 50 μg/ml) to PBMC culture. Negative control was made from PBMC culture without propolis extract for comparison. The cells were incubated for 48 hours in a 5% CO2 incubator at 37ºC. The proportion of CD8+ and CD4+CD25+ regulatory T cells that expressed granzyme were measured by flow cytometry. Results: The results obtained that the level of CD4+, CD4+CD25+, and CD8+ T cells were not markedly depleted in propolis treatment in all doses compared to control group. The percentage of CD8+Granzyme+ and CD4+CD25+Granzyme+ were significantly different in groups of propolis treatment compared to control group. Conclusion: In summary, propolis extract may serve as an immunomodulatory agent in cervical cancer patients. Propolis extract could modulate the expression CD4+CD25+ T cells expressing granzyme and CD8+ T cells expressing granzyme while it has no effect on CD4+, CD4+CD25+, and CD8+ T cells. Keywords: CD8+ T cells, CD4+CD25+ regulatory T cells, granzyme, propolis

Authors and Affiliations

Zauhani Kusnul, Pudji Rahayu, Muhaimin Rifai, Edi Widjajanto

Keywords

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  • EP ID EP449819
  • DOI 10.25002/tji.2017.544
  • Views 129
  • Downloads 0

How To Cite

Zauhani Kusnul, Pudji Rahayu, Muhaimin Rifai, Edi Widjajanto (2017). Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells. Turkish Journal of Immunology, 5(1), 13-19. https://europub.co.uk/articles/-A-449819