IMMUNOPHENOTYPIC FEATURES OF T CELL- ACUTE LYMPHOBLASTIC LEUKEMIA IN SUDAN
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 7
Abstract
Background: Acute lymphoblastic leukemia (ALL) is the most common type of neoplastic disorder diagnosed in childhood. It is the cause of one third of all pediatric malignancies. Immunophenotyping has become extremely important not only in diagnosis and sub classification of T-ALL but also in the detection of the minimal residual disease. Immunophynotypic pattern of T-ALL in Sudanese patients have not been addressed before. This study was designed to characterize immunophenotypic patterns of T-ALL in Sudanese patients. Multiparameter flow cytometry and CD45/SSC gating were used to analyze the surface and cytoplasmic antigen expressions in 50 cases of T-ALL during the period January 2017 to April 2017 at Flowcytometry laboratory-Khartoum, Sudan. The following antigens: CD45, HLA-DR, CD34, CD1a, CD2, surface and cytoplasmic CD3, CD4, CD5, CD7 and CD8 were used. Results: All T-ALL blasts expressed CD45 with no significant differences between different T-ALL subtypes, whereas, CD34 showed different expressions. cCD3 (cytoplasmic) and sCD3 (surface) were studied among the blast population, having mean positivity of 77.7% and 19.2%, respectively in all T-ALL subtypes, collectively. CD1a was found to have higher positivity among cortical T-ALL, with mean positivity of 81.7%. No significant correlation between gender, and age to T-ALL subtype (P value = 3.75, P value = 5.38), respectively. CD5 and CD2 were mostly expressed in pre T-ALL with mean positivity of (92.8%) and (55.6%), respectively. CD5 and CD2 were not expressed in pro T-ALL and have a mean negativity of (20.1%), (7.3%); respectively. sCD3 was highly expressed in cases of mature T-ALL with mean (87.7%). Interestingly, our results revealed that cortical T-ALL is the most predominant subtype among the Sudanese patients (68%). Conclusion: In summary, we provide immunophenotype Flow cytometric pattern of T-ALL in Sudanese patients and our findings are shown to be similar to the main immunophenotypic features published in literature. In addition, the analysis of combining the patterns and intensity of antigen expression is expected to improve the diagnosis of T-ALL in Sudan.
Authors and Affiliations
Enaam M. A. A. Hussein
Keywords
Related Articles
- EP ID EP628697
- DOI -
- Views 155
- Downloads 0
DYSPHAGIA CAUSES AND ITS RISK FACTORS IN MAK NIMER HOSPITAL NORTH OF SUDAN
This prospective hospital-based study done in Shendi and Elmak Nimer University Hospitals during the period from 1-6-2006 to 31-12-2008. It includes 79 patients who had dysphagia, 15 cases (19%) who had neurological dysp...
COMPARISON BETWEEN THE FERTILITY RATE AMONG SELECTED GROUP OF URBAN AND RURAL SUDANESE MALES APPLYING HYALURONAN BINDING ASSAY METHOD
Background: Sperm hyaluronan binding may be an indicator of which sperm are most likely to produce a viable pregnancy. For example, mature, hyaluronan-binding sperm are essentially free of cytoplasmic inclusions. This is...
ROLE OF MXA AND NEUTRALIZING ANTIBODIES IN EARLY RESPONSE TO INTERFERON TREATMENT IN HEPATITIS C EGYPTIAN PATIENTS
Pegylated interferon-alpha (PEG-IFN-α) and ribavirin (RBV) treatment does not succeed to attain a sustained virological response (SVR) in about 20-50% of patients with chronic hepatitis C virus (HCV) infection. It has be...
SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN E VIA FORMATION OF GOLD COMPLEX
Two different methods were used for the determination of vitamin E (Tocopherol) via complex formation with gold ions in solutions using UV - VIS spectrophotometry and flame atomic absorption spectrophotometry (AAS). The...
COMPARATIVE STUDY OF THE BIOSYNTHESIS OF PROTEINS OF RAT LIVER MITOCHONDRIA AT PHYSIOLOGICAL AGING AND THE MODEL OF “ACCELERATED AGING”, INFLUENCE ON THIS INDICATOR OF SOME PLANT PREPARATIONS
It has been determined that quercetin and its supramolecular complex have the ability to stimulate the translation processes in mitochondria of rat liver in the model of “accelerated aging”, as well as rats of different...