Immunoregulatory Effects of Human Amnion Epithelial Cells on Natural Killer and T Cells in Women with Recurrent Spontaneous Abortion (RSA)
Journal Title: Turkish Journal of Immunology - Year 2019, Vol 7, Issue 1
Abstract
Introduction: Unexplained Recurrent Spontaneous Abortion (URSA) is the most common immunological complication during pregnancy. It has been found that the cells such as human amnion epithelial cells (hAECs) have the potency to modulate immune responses in vitro and in vivo. In the present study, we assessed the immunomodulatory effect of hAECs on NK cells and T cells in women with URSA. Materials and Methods: Peripheral Blood mononuclear cells (PBMC) were obtained from 14 URSA patients and co-cultured with isolated hAECs. NK cells and T cells were identified using anti-CD56 and anti-CD3 monoclonal antibodies (mAb). The expression of the activating receptor CD69 and the degranulation marker CD107a on NK cells and T cells were detected using specific mAb and analyzed by flow cytometry. Results: We found that CD69 activating receptor expression on NK cells and T cells was significantly decreased by incubation with hAECs in a dose-dependent manner (p=0.049). Also, the degranulation marker CD107a was significantly downregulated on NK cells and T cells following incubation with hAEC (p=0.003). Conclusion: Our results suggest hAECs have immune regulatory effects on activation and cytotoxicity of NK and T cells. Potential therapeutic application of hAECs for dysregulated NK and T cells immunity should be investigated in the future.
Authors and Affiliations
Fahimeh KHADEM, Nafiseh ESMAEIL, Abbas REZAEI, Hossein MOTADAYEN, Behnaz KHANI
Keywords
Related Articles
- EP ID EP668778
- DOI 10.25002/tji.2019.991
- Views 102
- Downloads 0
Prevalence of HLA-DQ*02 and HLA-DQ*08 in Patients with Celiac Disease in Eastern Anatolia and the Diagnostic Role of HLA-DQ*02 and HLA-DQ*08 Genotyping
Introducton: Celiac disease (CD) is diagnosed with serological tests and small bowel biopsy. There is a strong link between CD and human leukocyte antigens (HLA). In this study, we aimed to determine the role of HLA alle...
Levels of IL-12, IL-17, and LL-37 in Acne Vulgaris
Introduction: Acne vulgaris (AV) is a chronic inflammatory disease of the pilosebaceous unit with a multifactorial pathogenesis, which includes colonization of Propionibacterium acnes (P. acnes). Increased P. acnes colon...
Effect of Ophiocephalus striatus Extract on the Levels of TNF-α, CRP, Leptin, Adiponectin, and COPD Assessment Test (CAT) Score in Stable COPD Patients with Muscle Wasting
Introduction: Muscle wasting is an extrapulmonary manifestation that occurs in 20–40% of patients with Chronic Obstructive Pulmonary Disease (COPD). The purpose of this study is to confirm whether nutrition support can i...
Effect of Monoclonal Antibody to Human Zona Pellucida 3 in Luteineizing Hormone Receptor Expression, Estradiol Levels and de Graaf Follicles Quantity in Mice
Objective: This research aimed to evaluate effect of monoclonal antibody to human Zona Pellucida 3 (Mab-hZP3) on luteineizing hormone (LH) receptor expression, estradiol levels and de Graaf follicles quantity of mus musc...
Bir Eğitim ve Araştırma Hastanesinde İFA Yöntemiyle Çalışılan Otoantikor Sonuçlarının Retrospektif Olarak Değerlendirilmesi
Retrospective Evaluation of Results of Autoantibodies Detected by IFA in a Training and Research Hospital Introduction: The present study evaluated the results of indirect fluorescent antibody (IFA) method performed in...