Impact of Marjoram (Origanum marjorana L.) Essential Oil on Some Virulence Factors and DNA Integrity of Multidrug Resistant Klebsiella pneumoniae
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 3
Abstract
Infectious diseases represent an important cause of morbidity and mortality among the populations, particularly in developing countries. Therefore, pharmaceutical companies have been motivated to develop new antimicrobial drugs in the recent years especially due to the constant emergence of microorganisms resistant to conventional antimicrobials. The current study aims to evaluate the antimicrobial efficacy of some essential oils (EOs) against multidrug resistant (MDR) K. pneumoniae. Antibiotic susceptibility profile of 15 K. pneumoniae isolates was performed against 14 antibiotics and 14 essential oils. The effect of the most potent EO against the chosen isolate regarding some virulence factors and DNA fragmentation was studied. Among all the tested antibacterial agents, MEO was the most effective one. Gas chromatography/mass spectrometry of MEO revealed that, terpinen-4-ol was the major constituent followed by γ-terpinene. Treatment using MEO led to a significant reduction in biofilm formation, complete killing within 4 hours, change in membrane permeability and reduction of capsular layer of the tested cells. Also, 2 MIC of MEO caused the highest damage of bacterial DNA. This study strongly suggests that MEO possesses antibacterial properties and induces DNA degradation. These results were correlated with chemical composition of the tested oil.
Authors and Affiliations
El- Tablawy, S. Y1, Araby, E. 2
Keywords
Related Articles
- EP ID EP388916
- DOI 10.9790/3008-1203016371
- Views 65
- Downloads 0
Comparative Study On Antimicrobial Susceptibility In Clinical Isolates At A Tertiary Care Teaching Hospital In South India
: Antimicrobial resistance is an issue of great significance for public health at global level. The purpose of study is to determine the susceptibility patterns of microrganisms to antibiotics and the prevalence of antib...
Effects of Industrial Waste on Genetic Variability of Pangasius hypophthalmus in Bangladesh
The effects of industrial effluents on genetic variability of Thai Pangas, Pangasius hypophthalmus were examined by seven RAPD primers in this research. Pangasius hypophthalmus were collected from pollution free water bo...
Dermatoglification Analysis for Selection and Training of Sports Talents
The term sports talent can be defined as the individual who, through his inherited and acquired conditions, has a special ability for sports performance, above the average of the general population. Fingerprints are gene...
Histopathological Study about effect of T.verrucosum on skin of the rabbits and treated by yellow sap and gel of Aloe vera
Background : The dermatophytesare taxonomically related fungi causing different skin infections referred to as tineas in man or ringworm in man and animals . Objective:This study was established to investigate theof effe...
In vitro Evaluation of Antifungal Activity of Leptadenia arborea against caused to tinea capitis
The present study was designed to investigate antifungal activity of Leptadenia arborea (Forssk) Schweinf.; (Ascolepidaceae) (leaves and stem). The ethanolic of leave and stem plant extract were seasoned against standard...