Impact of Media and Different Cytokinins Concentrations on in Vitro Shoot Multiplication of Persian Walnut (Juglans regia L.)
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 2
Abstract
Walnut (Juglans regia L.) is one of the important nut trees in Iran and has a wide distribution and diversity in this country. Walnut propagation especially micropropagation has some problems. This study was carried out in order to investigate shoot proliferation of two genotypes (Z60 and Z63) of J. regia L. on three culture media (MS, WPM and DKW) containing different concentrations of diverse cytokinins. Cytokinins were BA, TDZ, Zeatin, Kinetin, at 0.0, 0.2, 0.4, 0.6, 0.8, mgl-1 combined with 0.01 mgl-1 IBA. Results showed that DKW medium was the best medium for the most of investigated parameters for walnut micropropagation. The highest number of shoots with callus (%) obtained in 0.8 mgl-1 TDZ. Media containing 0.8 mgl-1 BA produced the highest number of multiple shoots/explant and the highest number of leaves. Also, the longest shoot length (cm) obtained from media containing 0.8 mgl-1 Zeatin and also the highest numbers of nodes obtained from media containing Zeatin. High concentrations of BA produced explants with high shoot fresh weights and high concentrations of TDZ lead to produce explants with high callus fresh weights.
Authors and Affiliations
Mohamad Bagher Yari, Mansour Gholami and Iraj khazaei
Keywords
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