Impact of Media and Different Cytokinins Concentrations on in Vitro Shoot Multiplication of Persian Walnut (Juglans regia L.)
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 2
Abstract
Walnut (Juglans regia L.) is one of the important nut trees in Iran and has a wide distribution and diversity in this country. Walnut propagation especially micropropagation has some problems. This study was carried out in order to investigate shoot proliferation of two genotypes (Z60 and Z63) of J. regia L. on three culture media (MS, WPM and DKW) containing different concentrations of diverse cytokinins. Cytokinins were BA, TDZ, Zeatin, Kinetin, at 0.0, 0.2, 0.4, 0.6, 0.8, mgl-1 combined with 0.01 mgl-1 IBA. Results showed that DKW medium was the best medium for the most of investigated parameters for walnut micropropagation. The highest number of shoots with callus (%) obtained in 0.8 mgl-1 TDZ. Media containing 0.8 mgl-1 BA produced the highest number of multiple shoots/explant and the highest number of leaves. Also, the longest shoot length (cm) obtained from media containing 0.8 mgl-1 Zeatin and also the highest numbers of nodes obtained from media containing Zeatin. High concentrations of BA produced explants with high shoot fresh weights and high concentrations of TDZ lead to produce explants with high callus fresh weights.
Authors and Affiliations
Mohamad Bagher Yari, Mansour Gholami and Iraj khazaei
Keywords
Related Articles
- EP ID EP32537
- DOI -
- Views 285
- Downloads 0
Foliar applications of gibberellic acid and benzyladenine increase vines of ‘White Butterfly’ Syngonium podophyllum
Syngonium podophyllum ‘White Butterfly’ is a species of species of Araceae, and commonly cultivated as a houseplant. A pot experiment was conducted to evaluate the effect of foliar spray of gibberellic acid (GA3) and b...
Safety and Nutritional Comparison of Fresh, Cooked and Frozen Mushroom (Agaricus bisporus)
As the consumption of different forms of mushrooms has been increased remarkably in recent years in Iran due to the high price of red meat, fish and other proteins , this research builds upon: Investigation the Effect...
Response of silicate fertilizer effects, rice husk and rice husk ash on rice paddy growth an seed yield (Shiroodi cultivar) in pot condition
Silicon is one of the important and necessary elements for rice growth, which has useful effects on rice growth. In shortage of this element, the plant goes to nutritional tribulation and we will be witness of discorda...
Micropropagation of Phalaenopsis amabilis cv. Cool 'Breeze' with using of flower stalk nodes and leaves of sterile obtained from node cultures
In this study we tried to produce this flower by micropropagation method with use of flower stalk nodes and leaves of sterile which are obtained from node cultures as explant. We examined different experiments such as...
A preliminary study on cultivation of Iranian wildgrowing medicinal mushroom Lentinus tigrinus
Wild mushrooms have been known to provide a significant source of nutritional and medicinal bioactive compounds. However, the production of fruiting bodies or mycelia in a standardized quality remains a challenge in ut...