Impact of small chemical elicitors on the production of volatile metabolites by endophytic fungi Fusariumsp and Phomopsissp from Cameroonian medicinal plants
Journal Title: Scholars Academic Journal of Pharmacy - Year 2016, Vol 5, Issue 9
Abstract
Abstract:This study was designed to study the effect of small organic chemicals on the production of volatile metabolites byFusariumsp and Phomopsis sp. Fungi were cultured for 6 days at 25°C in static condition in potato dextrose broth (PDB), PDB supplemented with 1µM of 5-Azacytidine, 1% DMSO, 1% Ethanol, 1%Methanol, 1% Acetone, 1% Acetonitrile, 1% 1-butanol and 1% Chloroform respectively. The ethyl acetate extracts prepared were analyzed by Gas chromatography coupled with Mass spectrometry (GC-MS). The results suggest that the production of volatile metabolites by Phomopsissp and Fusariumsp is significantly affected by the chemicals used as elicitors. The production of Heptaethylene glycol; Z-10-Methyl-11-tetradecen-1-ol propionate; 1-butanol. 1-Di (tert-butyl) silyloxy-2-phenylethane; 1, 2-Butanediol and Linolenin, 1-mono by Phomopsissp were induced by chemicals. While, Tetraacetyl-d-xylonic nitrile; 1-hexadecanol; 2-methyl-, Tetradecane, 2,6,10-trimethyl-; Octadecanal; 2-bromo-,(2-phenyl-1,3-dioxolan-4-yl)methyl 9octadecenoate, cis-; Methyl glycolate, benzyldimethylsilyl ether; 4-Octadecenal; Methyltartronic acid; 1,2-butanediol; Hexa-hydro-farnesol; 5-octadecenal; Hexaethylene glycol; Butyl 9, 12-octadecadienoate, Butyl 9-octadecenoate; Stearin 1, 3-di; Mono-2-ethylhexyl adipate, 4-methoxy-3-(2-methoxy-6-oxo-1-cyclohexen-1-yl) phenyl acetate and Heptacosane were produced by Fusariumsp only when grown in presence of elicitors. These results suggests the capability these chemicals to stimulate the production of new secondary metabolites by Fusariumsp and Phomopsissp. Keywords:Fusariumsp, Phomopsissp, Small organic chemicals,GC-MS.
Authors and Affiliations
Toghueo KRM, Dinkar S, Fekam BF
Keywords
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