Improvement Germination and Conversion of Somatic Embryos and Production of Normal Plantlets in Ferula Assa-foetida L. (A Rare Medicinal Plant)
Journal Title: European Journal of Medicinal Plants - Year 2014, Vol 4, Issue 12
Abstract
Secondary somatic embryogenesis leads to the formation of abnormal somatic embryos and produces abnormal seedlings. Normal plants are difficult to obtain from these embryos, due to the asynchronous maturation of the embryogenic tissues and low germination and conversion rates. The effects of some media additives and different strengths of MS medium on germination and plantlet formation of in vitro derived somatic embryos of Ferula Assa-foetida were studied. The highest number of normal embryos was observed in MS medium containing 30g/l sucrose with 0.5% or 1% AC and in MS medium supplemented with PEG and 0.5% or 1% AC. The treatments of MS medium with 30g/l sucrose and 0.5% AC × MS medium containing sorbitol and MS medium containing PEG and 1% AC × ½ MS had maximum number of normal germinated embryos without secondary somatic embryogenesis (SSE). In some of the treatments the embryos were converted better than the others, such as; the interaction effect of MS medium with 30g/l sucrose and 0.5% AC× MS, MS medium with 30g/l sucrose × MS medium with glutamine. Using different strength of MS medium and presence of some media additives is effective on germination and conversion of somatic embryos into normal plantlet. Presence of Activated Charcoal in the culture medium can reduce secondary somatic embryogenesis.
Authors and Affiliations
Shirin Roozbeh, Mahmoud Otroshy
Keywords
Related Articles
- EP ID EP350106
- DOI 10.9734/EJMP/2014/10215
- Views 79
- Downloads 0
In vitro Anthelmintic Activity of Bidens pilosa Linn. (Asteraceae) Leaf Extracts against Haemonchus contortus Eggs and Larvae
Haemonchus contortus is a major health and welfare problem for small ruminants, responsible for economic losses through reduced productivity and increased mortality. The in vitro efficacy of Bidens pilosa was determined...
Microbiological Contamination and Anti-bacterial Traits of Common Oral Herbal Medicinal Products within Dhaka Metropolis
Aims: Present study endeavored to examine the growth and survival of microorganisms within 6 categories of oral herbal medicines commonly used by the community within Dhaka metropolis. Methodology: Samples were analyzed...
Evaluation of Anticonvulsant Activity of Aqueous Leaf Extract of Telfairia occidentalis in Mice
Aims: This work evaluated the anticonvulsant effect of aqueous leaf extract of Telfairia occidentalis in Mice. This was to validate the use of T. occidentalis in the treatment of sudden attack of convulsions in folkloric...
Paulownia as a Medicinal Tree: Traditional Uses and Current Advances
Paulownia is one of the most useful and sought after trees, in China and elsewhere, due to its multipurpose status. Though not regarded as a regular medicinal plant species, various plant parts (leaves, flowers, fruits,...
Utilization of Wild Plants for Medicinal Purposes in Selected Tiv Communities of Benue State, Nigeria: An Ethnobotanical Approach
Aims: An ethnobotanical survey of wild plants used for the treatment of ailments was carried out among randomly selected sub-tribes in Tiv communities of Benue State. Study Design: Group Interviews were carried out with...