IN VITRO ANTIBACTERIAL ACTIVITES, SAFETY STUDIES AND PHYTOCHEMICAL SCREENING OF DREGEA SCHIMPERI CLARK (ASCLEPIADACEAE) EXTRACTS
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 7
Abstract
Plants synthesize phytochemical compounds for protection against environmental stress and diseases. Some of the phytochemicals are used for curative and preventive medicine. Over 90% plant materials are used in traditional medicine to treat diseases in human and veterinary diseases. Since a great percentage of plant materials used lack sufficient scientific data to back their healing claims, the current study focused on the evaluation of antimicrobial, cytotoxic and phytochemical properties of methanolic and water extracts of Dregea schimpheri leaves and husks. D. schimperi has many ethnomedical claims including the management of infectious diseases. Disc diffusion method was used to assay for antimicrobial activities of the methanolic and water extracts of leaves and husks against Staphylococcus aureus, Micrococcus luteus, Bacillus pumilus, Pseudomonas aerugnosa and Escherichia coli. Cytotoxicity of the extracts was determined by use of brine shrimp lethality test. Qualitative phytochemical screening of the husks and leaves was performed with standard phytochemical tests. Antibacterial results were tabulated as mean zone of inhibition ± SEM. LC50 values for brine shrimp lethality test were estimated using Graphed Prism Version 5 statistical software. Phytochemical screening observations were also tabulated. Most extracts exhibited antibacterial activity against S. aureus, M. luteus, B. pumilus, E. coli and P. aurignosa. Water and methanol husk extracts of D. schimperi were found to be cytotoxic (LC50 ˂100μg/ml) while all leaf extracts had moderate to low toxicity. Phytochemical screening revealed presence of alkaloids, phenols, tannins in both leaf and husk powders. Saponins and anthraquinones were present in leaf extracts but absent in the husks. The antibacterial and cytotoxic activities are attributed to the presence of these secondary metabolites. Further studies aimed at isolating the bioactive compounds with antibacterial and cytotoxic properties are recommended. In vitro and in vivo toxicity studies are also necessary.
Authors and Affiliations
Onyancha Jared M.
Keywords
Related Articles
- EP ID EP617772
- DOI -
- Views 163
- Downloads 0
REVIEW OF PARADI GUNA AND ITS UTILITY IN AYURVEDA CHIKITSA
Ayurveda is such a knowledge that indicate the appropriate and inappropriate, happy or sorrowful conditions of living beings, what is auspicious or inauspicious for longevity as well as good measures of life itself. On t...
B. RATHINA NAICKER & SONS – ONE OF THE PIONEERS IN SIDDHA LITERATURE PUBLICATION
Siddha system of medicine is having vast medical knowledge of the ethnic Tamil People. This medical wisdom was passed through oral tradition through the ages and later converted into written script in ancient times. A lo...
RELIABILITY OF COLPOSCOPY DURING PREGNANCY
The high specificity of colposcopy in pregnancy for the diagnosis of invasive carcinoma allows selecting with reasonable certainty patients to be subjected to diagnostic excision in case of suspected colposcopic invasion...
DIAGNOSTIC METHOD OF CHRONIC MERCURY TOXICITY AND ITS AYURVEDIC MANAGEMENT
Long term hazards due to prolong persistent continuous exposure of mercury causes chronic cumulative toxicity in human beings. Heavy industrialization, gold mining, utilization of fishes as a food containing methyl mercu...
A REVIEW ON “PHARMACOGNOSTIC, PHYTOCHEMICAL STUDY OF AZADIRACHTA INDICA” BARK EXTRACTS
Azadiracta indica A. Juss (Meliaceae) commonly known as Neem, is found throughout India and is known to have many wonderous properties from ancient times. These A. indica shows different medicinal properties like antiulc...