IN VITRO ANTIBACTERIAL ACTIVITY OF CAMELLIA SINENSIS AND ANDROGRAPHIS PANICULATA AGAINST VANCOMYCIN RESISTANT ENTEROCOCCI
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 2
Abstract
The herbal extracts of Camellia sinensis (Cs) and Andrographis paniculata (Ap) with standard F/P ratio (flavonoid/ polyphenol) of 0.26-0.27 were used for evaluation of anti-microbial efficacy against Vancomycin Resistant Enterococci (VRE). The bioactivity fingerprints useful for standardizing both extracts were found to be coherent with respect to their Diphenyl picryl hydrazyl radical scavenging potential (IC50 = ~ 30µg/ml). In vitro efficacy analysis of Cs and Ap extracts using exponentially growing culture of VRE revealed Minimum Inhibitory Concentration (MIC) as 200µg/mL and 50µg/mL respectively. The comparative analysis of their Minimum Bactericidal Concentration (MBC) revealed that 5X concentration of Cs as against 10X concentration of Ap was required to achieve zero microbial count. Such analysis indicated that both extracts would be useful for future analysis. In vivo pre-clinical evaluation studies are underway for utilization of identified herbal leads for trials on humans.
Authors and Affiliations
Pallavi Thakur, Raman Chawla, Ankita Singh Chakotiya, Raghav Nagpal, Ankit Tanwar, Rajeev Goel, Alka Narula, Rajesh Arora, Rakesh K. Sharma
Keywords
Related Articles
- EP ID EP625061
- DOI -
- Views 66
- Downloads 0
SYNTHESIS, ANTICONVULSANT AND NEUROTOXICITY SCREENING OF SOME NOVEL 2, 5-DISUBSTITUTED - 1, 3, 4 – OXADIAZOLE DERIVATIVES
A series of 2, 5-disubstituted - 1, 3, 4 – oxadiazoles (4a-o) were synthesized on refluxing hydrazine carbothioamides with iodine and potassium iodide in ethanolic sodium hydroxide solution starting from methyl-3-amino-4...
A HIGHLY VALIDATED RP-HPLC METHOD DEVELOPMENT FOR THE SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN IN TABLET DOSAGE FORMS
A simple, accurate, precise method was developed for the simultaneous estimation of Dapagliflozin and Saxagliptin in Tablet dosage form. Chromatogram was run through Standard BDS C8 column (50 × 4.6 mm, 5m) The Mobile ph...
SAFETY, TOLERABILITY AND BIOEQUIVALENCE ASSESSMENT OF A DISPERSIBLE TABLET FORMULATION OF PYRAZINAMIDE
Pyrazinamide is a widely used drug for the treatment of tuberculosis. A 150 mg Dispersible Tablet (DT) formulation of the drug was prepared by Micro Labs, a generic drug company. The formulation was developed for easy ad...
ANTI-INFLAMMATORY ACTIVITY AND TOTAL FLAVONOID CONTENT OF AEGLE MARMELOS SEEDS
The anti-inflammatory effect of aqueous and methanol extracts of Aegle marmelos seeds was evaluated using carrageenan induced paw edema and cotton pellets induced granuloma in rats. To prove the dependency of pharmacolog...
SOLUBILITY ENHANCEMENT OF ARIPIPRAZOLE BY SOLID-SELF EMULSIFYING DRUG DELIVERY SYSTEMS
Self-emulsifying drug delivery systems are a promising approach for the formulation of drug compounds with poor aqueous solubility. The main objective of this work was to formulate liquid and solid-self emulsifying drug...