IN VITRO ANTIBACTERIAL ACTIVITY OF CAMELLIA SINENSIS AND ANDROGRAPHIS PANICULATA AGAINST VANCOMYCIN RESISTANT ENTEROCOCCI
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 2
Abstract
The herbal extracts of Camellia sinensis (Cs) and Andrographis paniculata (Ap) with standard F/P ratio (flavonoid/ polyphenol) of 0.26-0.27 were used for evaluation of anti-microbial efficacy against Vancomycin Resistant Enterococci (VRE). The bioactivity fingerprints useful for standardizing both extracts were found to be coherent with respect to their Diphenyl picryl hydrazyl radical scavenging potential (IC50 = ~ 30µg/ml). In vitro efficacy analysis of Cs and Ap extracts using exponentially growing culture of VRE revealed Minimum Inhibitory Concentration (MIC) as 200µg/mL and 50µg/mL respectively. The comparative analysis of their Minimum Bactericidal Concentration (MBC) revealed that 5X concentration of Cs as against 10X concentration of Ap was required to achieve zero microbial count. Such analysis indicated that both extracts would be useful for future analysis. In vivo pre-clinical evaluation studies are underway for utilization of identified herbal leads for trials on humans.
Authors and Affiliations
Pallavi Thakur, Raman Chawla, Ankita Singh Chakotiya, Raghav Nagpal, Ankit Tanwar, Rajeev Goel, Alka Narula, Rajesh Arora, Rakesh K. Sharma
Keywords
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