In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda
Journal Title: Microbiology Research Journal International - Year 2015, Vol 8, Issue 3
Abstract
Aims: To determine antibacterial activity of Ocimum suave essential oils against bacterial uropathogens. Study Design: A cross sectional and experimental study. Place and Duration of Study: Six selected hospitals in Bushenyi District, Uganda between June 2012 and July 2013. Methodology: Clean catch midstream urine samples were collected and inoculated on Cystine Lysine Electrolyte Deficient (CLED) agar. The plates were incubated at 37°C for 24hrs to 48hrs. The O. suave essential oils were extracted by hydrodistillation of leaves for 4hrs using a Clevenger apparatus. The oil was collected and dried over anhydrous sodium sulphate (Na2SO4) and kept at 4°C till further use. The antimicrobial activity of O. suave essential oils against isolates was determined by agar well method. The MIC of O. suave essential oil extract was carried out by micro-broth dilution method. Results: Of the three hundred (300) midstream urine samples collected, 67(22.33%) had significant bacterial growth. Escherichia coli is the most common isolate (61.19%, n = 41). The essential oil from O. suave showed activity against isolates of E. coli, K. pneumoniae, S. aureus, E. feacalis, M. morganii, Citrobacter species, Enterobacter species and P. aeruginosa with mean zone of inhibition (ZI) ranging from 10-22 mm. The essential oils had no inhibitory activity on Acinetobacter species. The minimum inhibitory concentration (MIC) for O. suave essential oils ranged from 0.78 to 22 µg/ml. This study showed that O. suave essential oils had MIC value of 0.78 µg/ml against S. aureus and MIC values ranging from 3 to 22 µg/ml against the other tested isolates. Conclusion: The most common uropathogen was E. coli (61.19% n = 41). O. suave essential oils exhibited antibacterial activity against majority of the uropathogens, except Acinetobacter species, mean ZI of 10-22 mm and MIC of 0.78 – 22 µg/ml.
Authors and Affiliations
Julius Tibyangye, Matilda Angela Okech, Josephat Maniga Nyabayo, Jessica Lukanga Nakavuma
Keywords
Related Articles
- EP ID EP354481
- DOI 10.9734/BMRJ/2015/17526
- Views 67
- Downloads 0
In vitro Antifungal Efficacies of Ethyl Acetate Fractions of Mitracarpus villosus from Abuja, Nigeria
Introduction: The use of medicinal plants in the treatment of diseases is as old as man. The development of synthetic (orthodox) drugs led to a decline in the use of herbs however in the past few decades, there has been...
Microbiological Quality of Machine Sealed Sachet Water Produced and Sold in the Tamale Metropolis, Ghana
The microbiological quality of machine sealed sachet water produced and sold in the Tamale Metropolis, Northern Region of Ghana was investigated. Eighteen (18) different sachet water brands produced and sold in Tamale we...
Alkylsulphatase Activity of Fungi Isolated from Rivers Contaminated with Surfactants in Akure, Nigeria
Aims: To isolate, characterize and identify surfactant degrading fungi from selected rivers in Akure, Nigeria and also to compare and quantify the biodegrading potentials of each of the fungal isolates. Plac...
Identification of Ascitic Fluid Bacterial Pathogens in Spontaneous Bacterial Peritonitis in Nile Delta and Its Impact on Clinical Outcome of these Patients
Aims: This study aimed to identify ascetic fluid bacterial pathogens and their antibiotic resistance profile in Spontaneous Bacterial Peritonitis (SBP) patients in Nile delta and its impact on the clinical outcome of the...
A Peculiar Phenomenon of Cold-shocked Bacteria Recovered on Sano- Gam Media
This study was conducted in order to display the peculiar appearance of bacteria which were recovered on Sano-Gam Media. The studied bacteria were Escherichia coli, Salmonella spp., Staphylococcus aureus, Proteus vulgari...