In vitro antimicrobial activity and determination of essential metal and ash value contents of Trichodesma zeylanicum
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2013, Vol 2, Issue 3
Abstract
Infectious diseases are responsible for morbidity and mortality of millions each year especially in developing countries. Microbial drug resistance is a serious problem for current effective antimicrobial agents necessitating the search for new antimicrobial agents from natural sources including plants. Trichodesma zeylanicum plants is used for medicinal and nutrition purposes. The objective of this study was to confirm the antimicrobial activity of T.zeylanicum leaves against selected pathogenic microbes, determine essential metal and ash content and detect the presence of nitrate and calcium oxalate. The crude aqueous methanolic extract was prepared from the dry powdered leaves of T.zeylanicum by maceration at room temperature. Eight bacterial and five fungi were used in the antimicrobial testing employing broth micro dilution method. Atomic Absorption Spectrophotometer was used for metal analysis. The crude extract of T.zeylanicum leaves exhibited activity against S.aureus, P.aeruginosa, S typhi and the dermatophyte T.mentagrophyte with MIC value of 1.25 mg/ml. The content of four essential minerals obtained are; iron (58.53 mg/kg), manganese (47.51 mg/kg), magnesium (3.43 mg/kg), copper (3.28 mg/kg) and ash value of 23.46%. Microscopic and qualitative chemical analysis studies revealed the presence abundant calcium oxalate crystal and nitrate respectively. Both antifungal and antibacterial activity support the traditional use of T.zeylanicum leaves against scalp fungal infection which is normally accompanied with secondary infection due to bacteria. Presence of essential elements justifies their nutritional potential but safety issues need to be addressed. This is the first study on the plant T.zeylanicum with regard to antifungal activity and metal analysis. Detail study is recommended to isolate bioactive principles and establishment of the complete nutritional and mineral profiles and safety.
Authors and Affiliations
Sheila M. Maregesi
Keywords
Related Articles
- EP ID EP411963
- DOI -
- Views 94
- Downloads 0
The study on effect of self auditing the prescriptions in a polyclinic
Introduction Prescription audit help us to monitor, evaluate and suggest necessary modifications in the prescribing practices to improve the quality of prescriptions. Our objective was to do the prescription audit of pa...
Evaluation of hypoglycaemic activity of shilajit in alloxan induced diabetic rats
Background Diabetes mellitus is a potentially morbid condition with high prevalence worldwide. This disease constitutes a major health concern affecting about 2.8% of the global population. Currently available synthetic...
Formulation and evaluation of fast dissolving film of meloxicam
Fast dissolving dosage forms have acquired great importance in pharmaceutical industry. The aim of this study was to formulate and characterize fast dissolving film containing meloxicam (MLX) for oral use. In this study,...
A study on evaluation of hypothyroidism associated with pregnancy women
Maternal hypothyroidism may place the mother at an increased risk of adverse obstetrical outcomes. Untreated hypothyroidism is associated with increased risk for pre-eclampsia, low birth weight, placental abruption, mi...
A comparative study between efficacy and safety of telmisartan (ARB) and enlapril (ACEI) in hypertensive patients
Background Hypertension is the most common cardiovascular disease that affects about 1 billion individuals worldwide1. It is defined conventionally as sustained increase in blood pressure ≥ 140/90mmHg. In India the p...